Rożyński Maciej, Demska-Zakęś Krystyna, Sikora Agnieszka, Zakęś Zdzisław
Department of Aquaculture, The Stanisław Sakowicz Inland Fisheries Institute, Oczapowskiego 10, 10-719, Olsztyn, Poland.
Department of Ichthyology, Faculty of Environmental Sciences, University of Warmia and Mazury, Oczapowskiego 5, 10-719, Olsztyn, Poland.
Fish Physiol Biochem. 2018 Jun;44(3):927-937. doi: 10.1007/s10695-018-0482-4. Epub 2018 Feb 23.
The aim of the study was to describe the course and timing of the different stages of anesthesia induced with Propiscin (etomidate) on juvenile European perch (experiment I) and to describe the effect of immersing specimens of this species had on selected hematological and biochemical parameters (experiment II). The study was conducted on material with body weights (BW) of 162.98 (experiment I) and 171.60 g (experiment II). In experiment I, general anesthesia was induced with two different anesthetic concentrations (1 or 2 ml l; anesthesia time 10 min). In experiment II, blood was drawn for hematological and biochemical analyses from the fish that had been exposed to anesthetic immersion baths with two different concentrations of Propiscin (1 and 2 ml l) and for different exposure times (3 and 10 min). Blood samples were collected immediately following immersion (0 h) and 24 h later (24 h). Specimens that were immersed at the higher concentration of anesthetic achieved subsequent stages of general anesthesia two times faster (P ≤ 0.05). However, during recovery, some statistically significant differences were observed, but these lasted only until stage I was achieved. Among the hematological parameters (0 h), significant differences were observed in hematocrit (HCT) and mean corpuscular volume (MCV), while among the biochemical determinations (0 h), statistically significant differences were noted in the concentrations of glucose, calcium, lactate, and ammonia. After 24 h, the levels of these parameters in all fish groups returned to initial values. The hematological and biochemical tests conducted permit concluding that the anesthetic tested, at the concentrations (1 and 2 ml l) and the exposure times of up to 10 min at which it was tested, is safe and can be used successfully to induce general anesthesia in perch.
本研究的目的是描述用丙泊酚（依托咪酯）对欧洲幼鲈进行麻醉时不同阶段的过程和时间（实验 I），并描述该物种样本浸入麻醉剂后对选定血液学和生化参数的影响（实验 II）。研究使用的材料体重分别为 162.98 g（实验 I）和 171.60 g（实验 II）。在实验 I 中，用两种不同的麻醉剂浓度（1 或 2 ml/l；麻醉时间 10 分钟）诱导全身麻醉。在实验 II 中，从暴露于两种不同浓度丙泊酚（1 和 2 ml/l）且暴露时间不同（3 和 10 分钟）的鱼中采集血液进行血液学和生化分析。在浸入后立即（0 小时）和 24 小时后（24 小时）采集血样。浸入较高浓度麻醉剂的样本达到全身麻醉后续阶段的速度快两倍（P≤0.05）。然而，在恢复过程中，观察到一些统计学上的显著差异，但这些差异仅持续到达到 I 阶段。在血液学参数（0 小时）中，血细胞比容（HCT）和平均红细胞体积（MCV）存在显著差异，而在生化测定（0 小时）中，葡萄糖、钙、乳酸和氨的浓度存在统计学上的显著差异。24 小时后，所有鱼组中这些参数的水平恢复到初始值。所进行的血液学和生化测试表明，所测试的麻醉剂在其测试的浓度（1 和 2 ml/l）以及长达 10 分钟的暴露时间下是安全的，可成功用于诱导鲈鱼的全身麻醉。
