使用双光子显微镜在细胞水平对心脏进行活体成像。

Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy.

作者信息

Matsuura Ryohei, Miyagawa Shigeru, Kikuta Junichi, Ishii Masaru, Sawa Yoshiki

机构信息

Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.

Department of Immunology and Cell Biology, Osaka University Graduate School of Medicine, Osaka, Japan.

出版信息

Methods Mol Biol. 2018;1763:145-151. doi: 10.1007/978-1-4939-7762-8_14.

DOI:10.1007/978-1-4939-7762-8_14

PMID:29476496

Abstract

Recent molecular approaches have provided deeper insight on heart failure. However, real-time in vivo cellular dynamics have not been satisfactorily visualized. Here, we present a detailed protocol for in vivo cellular imaging for visualization of the rat heart using two-photon microscopy.

摘要

最近的分子方法为心力衰竭提供了更深入的见解。然而，体内细胞的实时动态尚未得到令人满意的可视化。在此，我们展示了一种详细的方案，用于使用双光子显微镜对大鼠心脏进行体内细胞成像以实现可视化。