Matsumoto Sayaka, Kikuta Junichi, Ishii Masaru
Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan.
Methods Mol Biol. 2018;1763:137-143. doi: 10.1007/978-1-4939-7762-8_13.
The liver is a vital organ in the body. It has various essential functions, including detoxification, protein synthesis, and control of infection. Because of its medical importance, liver diseases such as hepatitis and cirrhosis can be crucial for an individual. Exploring dynamics of living cells in the liver would provide the clues for understanding the pathology. However, due to its technical difficulty, few studies have used intravital liver imaging. To resolve this, we have established a novel imaging system for visualizing liver cell dynamics in living animals.Herein we describe the methodology for visualizing the in vivo behavior of liver cells using intravital multiphoton microscopy. This approach will be useful for understanding the pathogenesis of liver disorders, as well as liver biology, in vivo.
肝脏是人体中的一个重要器官。它具有多种重要功能，包括解毒、蛋白质合成和感染控制。由于其医学重要性，诸如肝炎和肝硬化等肝脏疾病对个体可能至关重要。探索肝脏中活细胞的动态变化将为理解病理学提供线索。然而，由于技术难度，很少有研究使用活体肝脏成像。为了解决这个问题，我们建立了一种用于可视化活体动物肝脏细胞动态变化的新型成像系统。在此，我们描述了使用活体多光子显微镜可视化肝脏细胞体内行为的方法。这种方法将有助于在体内理解肝脏疾病的发病机制以及肝脏生物学。
