Schrader Michael
Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Germany.
Methods Mol Biol. 2018;1719:3-39. doi: 10.1007/978-1-4939-7537-2_1.
Peptidomics is the comprehensive characterization of peptides from biological sources mainly by HPLC and mass spectrometry. Mass spectrometry allows the detection of a multitude of single peptides in complex mixtures. The term first appeared in full papers in the year 2001, after over 100 years of peptide research with a main focus on one or a few specific peptides. Within the last 15 years, this new field has grown to over 1200 publications. Mass spectrometry techniques, in combination with other analytical methods, were developed for the fast and comprehensive analysis of peptides in proteomics and specifically adjusted to implement peptidomics technologies. Although peptidomics is closely linked to proteomics, there are fundamental differences with conventional bottom-up proteomics. The development of peptidomics is described, including the most important implementations for its technological basis. Different strategies are covered which are applied to several important applications, such as neuropeptidomics and discovery of bioactive peptides or biomarkers. This overview includes links to all other chapters in the book as well as recent developments of separation, mass spectrometric, and data processing technologies. Additionally, some new applications in food and plant peptidomics as well as immunopeptidomics are introduced.
肽组学主要通过高效液相色谱和质谱对生物来源的肽进行全面表征。质谱能够检测复杂混合物中的多种单个肽。在肽研究主要聚焦于一种或几种特定肽超过100年后，该术语于2001年首次完整出现在论文中。在过去15年里，这个新领域的相关出版物已超过1200篇。质谱技术与其他分析方法相结合，被开发用于蛋白质组学中肽的快速全面分析，并经过专门调整以实施肽组学技术。尽管肽组学与蛋白质组学密切相关，但与传统的自下而上蛋白质组学存在根本差异。本文描述了肽组学的发展，包括其技术基础的最重要实现方式。涵盖了应用于多种重要应用（如神经肽组学以及生物活性肽或生物标志物的发现）的不同策略。本综述包括与本书所有其他章节的链接以及分离、质谱和数据处理技术的最新进展。此外，还介绍了食品和植物肽组学以及免疫肽组学中的一些新应用。
