Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang 550081, China.
Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang 550081, China; State Key Laboratory of Rice Biology, Institution of Crop Science, Zhejiang University, Hangzhou 310058, China.
Genomics. 2019 Mar;111(2):142-158. doi: 10.1016/j.ygeno.2018.01.010. Epub 2018 Feb 21.
Tobacco (Nicotiana tabacum) serve as the top leading commercial, non-food, and model crop worldwide. Cyclic nucleotide-gated channels (CNGCs) are ligand-gated, calcium-permeable, divalent, cation-selective channels, involved in important biological functions. Here, we systematically characterized thirty-five CNGC genes in the genome of Nicotiana tabacum, and classified into four phylogenetic groups. Evolutionary analysis showed that NtabCNGC family of N. tabacum originated from the parental genome of N. sylvestris and N. tomentosiformis, and further expanded via tandem and segmental duplication events. Tissue-specific expression analysis showed that twenty-three NtabCNGC genes are involved in the development of various tobacco tissues. Subsequent RT-qPCR analyses indicated that these genes are sensitive towards external abiotic and biotic stresses. Notable performances were exhibited by group-I and IV CNGC genes against black shank, Cucumber mosaic virus, Potato virus Y, cold, drought, and cadmium stresses. Our analyses also suggested that NtabCNGCs can be regulated by phosphorylation and miRNAs, and multiple light, temperature, and pathogen-responsive cis-acting regulatory elements present in promotors. These results will be useful for elaborating the biological roles of NtabCNGCs in tobacco growth and development.
烟草（Nicotiana tabacum）是全球最重要的商业非粮食作物和模式作物。环核苷酸门控通道（CNGCs）是配体门控、钙离子通透、二价阳离子选择性通道，参与重要的生物学功能。在这里，我们系统地鉴定了烟草基因组中的 35 个 CNGC 基因，并将其分为四个系统发生组。进化分析表明，烟草 NtabCNGC 家族起源于野烟草和绒毛烟草的双亲基因组，并通过串联和片段重复事件进一步扩张。组织特异性表达分析表明，23 个 NtabCNGC 基因参与了各种烟草组织的发育。随后的 RT-qPCR 分析表明，这些基因对外源生物和非生物胁迫敏感。第一组和第四组 CNGC 基因在抗黑胫病、黄瓜花叶病毒、马铃薯 Y 病毒、冷胁迫、干旱胁迫和镉胁迫方面表现出显著的性能。我们的分析还表明，NtabCNGCs 可以受到磷酸化和 miRNA 的调控，并且启动子中存在多个光、温度和病原体响应的顺式作用调控元件。这些结果将有助于阐述 NtabCNGCs 在烟草生长和发育中的生物学作用。
