Yu Shizhou, Kakar Kaleem Ullah, Yang Zhixiao, Nawaz Zarqa, Lin Shifeng, Guo Yushuang, Ren Xue-Liang, Baloch Akram Ali, Han Dejun
Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang 550081, China; College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, People's Republic of China; State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, People's Republic of China.
Department of Biotechnology, Faculty of Life Sciences and Informatics, Balochistan University of Information Technology and Management Sciences, Quetta 87300, Pakistan.
Genomics. 2020 Mar;112(2):1404-1418. doi: 10.1016/j.ygeno.2019.08.010. Epub 2019 Aug 17.
Plant respiratory burst oxidase homolog (Rboh) gene family encodes the key enzymatic subunits of reactive oxygen species (ROS) production pathways, and play crucial role in plant signaling, development and stress responses. In present work, twenty genes were identified in Nicotiana tabacum Rboh family (NtabRboh) and classified into four phylogenetic groups (I-IV). Fourteen NtabRboh genes were positioned on ten chromosomes (i.e., Ch1, 2, 4, 7-11, 14 and 21), and six scaffolds. Synteny and evolutionary analysis showed that most of the NtabRboh genes have evolved from the genomes of the ancestor species (N. tomentosiformis and N. sylvestris), which afterwards expanded through duplication events. The promoter regions of the NtabRboh genes contained numerous cis-acting regulatory elements for hormones, plant growth, and different biotic and abiotic factors. The NtabRbohF gene transcript comprised target sites for wounding and stress responsive microRNAs: nta-miR166a-d, g and h. The transcript abundance of NtabRboh genes in different tissues reflected their important for plant growth and organ development in tobacco. RT-qPCR-assays demonstrated that the expression of NtabRboh genes are regulated by viral and bacterial pathogens, drought, cold and cadmium stress. The expression levels NtabRbohA, B and C were significantly up-regulated in "black shank and tobacco mosaic virus-inoculated susceptible and transgenic tobacco cultivars, showing that these genes play important roles in disease resistance.
植物呼吸爆发氧化酶同源物（Rboh）基因家族编码活性氧（ROS）产生途径的关键酶亚基，并在植物信号传导、发育和应激反应中发挥关键作用。在本研究中，在烟草Rboh家族（NtabRboh）中鉴定出20个基因，并分为四个系统发育组（I-IV）。14个NtabRboh基因定位在10条染色体（即Ch1、2、4、7-11、14和21）和6个支架上。共线性和进化分析表明，大多数NtabRboh基因是从祖先物种（绒毛状烟草和林烟草）的基因组进化而来的，随后通过复制事件进行了扩展。NtabRboh基因的启动子区域包含许多用于激素、植物生长以及不同生物和非生物因子的顺式作用调控元件。NtabRbohF基因转录本包含伤口和应激响应微小RNA（nta-miR166a-d、g和h）的靶位点。NtabRboh基因在不同组织中的转录丰度反映了它们对烟草植物生长和器官发育的重要性。RT-qPCR分析表明，NtabRboh基因的表达受病毒和细菌病原体、干旱、寒冷和镉胁迫的调控。NtabRbohA、B和C的表达水平在“黑胫病和烟草花叶病毒接种的易感和转基因烟草品种中显著上调，表明这些基因在抗病性中发挥重要作用。
