Pfeiffer Jana, Tarbashevich Katsiaryna, Bandemer Jan, Palm Thomas, Raz Erez
Institute of Cell Biology, Center for Molecular Biology of Inflammation, University of Münster, Von-Esmarch-Str. 56, 48149 Münster, Germany.
Institute of Cell Biology, Center for Molecular Biology of Inflammation, University of Münster, Von-Esmarch-Str. 56, 48149 Münster, Germany.
Dev Biol. 2018 Apr 15;436(2):84-93. doi: 10.1016/j.ydbio.2018.02.014. Epub 2018 Feb 22.
Zebrafish primordial germ cells (PGCs) constitute a useful in vivo model to study cell migration and to elucidate the role of specific proteins in this process. Here we report on the role of the heat shock protein Hsp90aa1.2, a protein whose RNA level is elevated in the PGCs during their migration. Reducing Hsp90aa1.2 activity slows down the progression through the cell cycle and leads to defects in the control over the MTOC number in the migrating cells. These defects result in a slower migration rate and compromise the arrival of PGCs at their target, the region where the gonad develops. Our results emphasize the importance of ensuring rapid progression through the cell cycle during single-cell migration and highlight the role of heat shock proteins in the process.
斑马鱼原始生殖细胞（PGCs）构成了一个有用的体内模型，可用于研究细胞迁移并阐明特定蛋白质在此过程中的作用。在此，我们报告热休克蛋白Hsp90aa1.2的作用，该蛋白的RNA水平在PGCs迁移过程中升高。降低Hsp90aa1.2的活性会减缓细胞周期进程，并导致迁移细胞中微管组织中心（MTOC）数量控制出现缺陷。这些缺陷导致迁移速率减慢，并影响PGCs到达其靶标，即性腺发育的区域。我们的结果强调了在单细胞迁移过程中确保细胞周期快速进程的重要性，并突出了热休克蛋白在此过程中的作用。
