Tabkhkar Narjes, Rabiei Babak, Samizadeh Lahiji Habibollah, Hosseini Chaleshtori Maryam
Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
Rice Research Institute of Iran (RRII), Agriculture Research Education and Extension Organization (AREEO), Rasht, Iran.
Biochem Genet. 2018 Aug;56(4):356-374. doi: 10.1007/s10528-018-9849-6. Epub 2018 Feb 24.
Drought is one of the major abiotic stresses, which hampers the production of rice worldwide. Informative molecular markers are valuable tools for improving the drought tolerance in various varieties of rice. The present study was conducted to evaluate the informative simple sequence repeat (SSR) markers in a diverse set of rice genotypes. The genetic diversity analyses of the 83 studied rice genotypes were performed using 34 SSR markers closely linked to the major quantitative trait loci (QTLs) of grain yield under drought stress (qDTYs). In general, our results indicated high levels of polymorphism. In addition, we screened these rice genotypes at the reproductive stage under both drought stress and nonstressful conditions. The results of the regression analysis demonstrated a significant relationship between 11 SSR marker alleles and the plant paddy weight under stressful conditions. Under the nonstressful conditions, 16 SSR marker alleles showed a significant correlation with the plant paddy weight. Finally, four markers (RM279, RM231, RM166, and RM231) demonstrated a significant association with the plant paddy weight under both stressful and nonstressful conditions. These informative-associated alleles may be useful for improving the crop yield under both drought stress and nonstressful conditions in breeding programs.
干旱是主要的非生物胁迫之一，它阻碍了全球水稻的产量。信息丰富的分子标记是提高各种水稻品种耐旱性的宝贵工具。本研究旨在评估一系列不同水稻基因型中的信息丰富的简单序列重复（SSR）标记。使用34个与干旱胁迫下（qDTYs）谷物产量的主要数量性状位点（QTL）紧密连锁的SSR标记，对83个研究的水稻基因型进行了遗传多样性分析。总体而言，我们的结果表明多态性水平较高。此外，我们在干旱胁迫和非胁迫条件下的生殖阶段对这些水稻基因型进行了筛选。回归分析结果表明，在胁迫条件下，11个SSR标记等位基因与植株稻谷重量之间存在显著关系。在非胁迫条件下，16个SSR标记等位基因与植株稻谷重量呈显著相关。最后，四个标记（RM279、RM231、RM166和RM231）在胁迫和非胁迫条件下均与植株稻谷重量表现出显著关联。这些信息丰富的相关等位基因可能有助于在育种计划中提高干旱胁迫和非胁迫条件下的作物产量。
