Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00 Brno, Czech Republic.
Institute of Chemistry, NAWI Graz, University of Graz, Heinrichstrasse 28/III, 8010 Graz, Austria; ARENA Arbeitsgemeinschaft für Ressourcenschonende & Nachhaltige Technologien, Inffeldgasse 21b, 8010 Graz, Austria.
Bioresour Technol. 2018 May;256:552-556. doi: 10.1016/j.biortech.2018.02.062. Epub 2018 Feb 16.
This work explores molecular, morphological as well as biotechnological features of the highly promising polyhydroxyalkanoates (PHA) producer Halomonas halophila. Unlike many other halophiles, this bacterium does not require expensive complex media components and it is capable to accumulate high intracellular poly(3-hydroxybutyrate) (PHB) fractions up to 82% of cell dry mass. Most remarkably, regulating the concentration of NaCl apart from PHB yields influences also the polymer's molecular mass and polydispersity. The bacterium metabolizes various carbohydrates including sugars predominant in lignocelluloses and other inexpensive substrates. Therefore, the bacterium was employed for PHB production on hydrolysates of cheese whey, spent coffee grounds, sawdust and corn stover, which were hydrolyzed by HCl; required salinity of cultivation media was set up during neutralization by NaOH. The bacterium was capable to use all the tested hydrolysates as well as sugar beet molasses for PHB biosynthesis, indicating its potential for industrial PHB production.
这项工作探讨了极具前景的聚羟基烷酸酯 (PHA) 生产者盐单胞菌的分子、形态和生物技术特征。与许多其他嗜盐菌不同，这种细菌不需要昂贵的复杂培养基成分，并且能够积累高达 82%细胞干重的高浓度胞内聚(3-羟基丁酸酯) (PHB)。最显著的是，除了 PHB 之外，调节 NaCl 的浓度还会影响聚合物的分子量和多分散性。该细菌可代谢各种碳水化合物，包括木质纤维素中占主导地位的糖和其他廉价的基质。因此，该细菌被用于在奶酪乳清、废咖啡渣、木屑和玉米秸秆的水解物上生产 PHB，这些水解物是通过 HCl 水解得到的；通过 NaOH 中和来设定培养介质所需的盐度。该细菌能够使用所有测试的水解物以及甜菜糖蜜进行 PHB 生物合成，表明其具有用于工业 PHB 生产的潜力。
