Koudelová J, Mourek J, Drahota Z
Physiol Bohemoslov. 1986;35(5):414-9.
The acetoacetic acid level in rat plasma was studied by Walker's method (Walker 1954) during ontogenesis, after 20 min exposure to altitude hypoxia (7,000 or 9,000 m) or after short-term (24 h) deprivation of food and water. Wistar rats of both sexes, bred at the authors' department, were employed. The results confirmed earlier findings on the acetoacetic acid level during ontogenesis (Drahota et al. 1963) in addition, it was found that the level of the given acid in the rats' blood fell steadily during the whole of the pre-weaning period. Short-term deprivation of food and water significantly raised the plasma acetoacetic acid level in 5- and 10-day-old and adult rats; the maximum absolute increase was found in the youngest age groups. Hypoxia corresponding to 7,000 m significantly raised the plasma acetoacetic acid level in 5- and 10-day-old rats, but not in adult animals. Hypoxia corresponding to 9,000 m raised it significantly in 5-day-old animals only. We associate these findings with the possible utilization of acetoacetic acid, as a suitable energy substrate, by certain tissues, in particular the tissues of the CNS, in the youngest organisms.
采用沃克方法（Walker，1954年）研究了大鼠血浆中乙酰乙酸水平在个体发育过程中的变化，以及在暴露于高原低氧环境（7000米或9000米）20分钟后或短期（24小时）禁食禁水后的变化。使用的是在作者所在科室饲养的雌雄Wistar大鼠。结果证实了早期关于个体发育过程中乙酰乙酸水平的研究结果（Drahota等人，1963年）。此外，还发现大鼠血液中该酸的水平在整个断奶前期持续下降。短期禁食禁水显著提高了5日龄、10日龄和成年大鼠血浆中乙酰乙酸水平；在最年幼的年龄组中发现了最大的绝对增加值。相当于7000米高度的低氧环境显著提高了5日龄和10日龄大鼠血浆中乙酰乙酸水平，但对成年动物没有影响。相当于9000米高度的低氧环境仅显著提高了5日龄动物的该水平。我们将这些发现与最年幼生物体中某些组织，特别是中枢神经系统组织可能将乙酰乙酸作为合适的能量底物加以利用联系起来。
