Zhou Yingchao, Xiao Hong, Wu Jianfei, Zha Lingfeng, Zhou Mengchen, Li Qianqian, Wang Mengru, Shi Shumei, Li Yanze, Lyu Liangkun, Wang Qing, Tu Xin, Lu Qiulun
Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology and Center for Human Genome Research, Huazhong University of Science and Technology, Wuhan, China.
Renmin Hospital of Wuhan University, Wuhan, China.
Cell Physiol Biochem. 2018;45(4):1541-1550. doi: 10.1159/000487690. Epub 2018 Feb 21.
BACKGROUND/AIMS: Diabetes mellitus (DM) has been demonstrated to have a strong association with heart failure. Conventional echocardiographic analysis cannot sensitively monitor cardiac dysfunction in type I diabetic Akita hearts, but the phenotype of heart failure is observed in molecular levels during the early stages.
Male Akita (Ins2WT/C96Y) mice were monitored with echocardiographic imaging at various ages, and then with conventional echocardiographic analysis and speckle-tracking based strain analyses.
With speckle-tracking based strain analyses, diabetic Akita mice showed changes in average global radial strain at the age of 12 weeks, as well as decreased longitudinal strain. These changes occurred in the early stage and remained throughout the progression of diabetic cardiomyopathy in Akita mice. Speckle-tracking showed that the detailed and precise changes of cardiac deformation in the progression of diabetic cardiomyopathy in the genetic type I diabetic Akita mice were uncoupled.
We monitored early-stage changes in the heart of diabetic Akita mice. We utilize this technique to elucidate the underlying mechanism for heart failure in Akita genetic type I diabetic mice. It will further advance the assessment of cardiac abnormalities, as well as the discovery of new drug treatments using Akita genetic type I diabetic mice.
背景/目的：糖尿病（DM）已被证明与心力衰竭密切相关。传统超声心动图分析无法灵敏地监测I型糖尿病阿基塔（Akita）小鼠的心脏功能障碍，但在早期阶段可在分子水平观察到心力衰竭的表型。
对雄性阿基塔（Ins2WT/C96Y）小鼠在不同年龄进行超声心动图成像监测，然后进行传统超声心动图分析和基于斑点追踪的应变分析。
基于斑点追踪的应变分析显示，糖尿病阿基塔小鼠在12周龄时平均整体径向应变发生变化，纵向应变降低。这些变化发生在早期阶段，并在阿基塔小鼠糖尿病性心肌病的整个进展过程中持续存在。斑点追踪显示，在遗传性I型糖尿病阿基塔小鼠糖尿病性心肌病进展过程中，心脏变形的详细而精确的变化是解耦的。
我们监测了糖尿病阿基塔小鼠心脏的早期变化。我们利用这项技术阐明I型糖尿病阿基塔小鼠心力衰竭的潜在机制。这将进一步推进对心脏异常的评估，以及利用I型糖尿病阿基塔小鼠发现新的药物治疗方法。
