Cellular and Molecular Cardiology Laboratory, Department of Physiology, University of Auckland, Auckland, New Zealand.
Radcliffe Department of Medicine, OCDEM, University of Oxford, Oxford, UK.
Sci Rep. 2023 Jul 31;13(1):12344. doi: 10.1038/s41598-023-39499-3.
Diastolic dysfunction is increasingly identified as a key, early onset subclinical condition characterizing cardiopathologies of rising prevalence, including diabetic heart disease and heart failure with preserved ejection fraction (HFpEF). Diastolic dysfunction characterization has important prognostic value in management of disease outcomes. Validated tools for in vivo monitoring of diastolic function in rodent models of diabetes are required for progress in pre-clinical cardiology studies. 2D speckle tracking echocardiography has emerged as a powerful tool for evaluating cardiac wall deformation throughout the cardiac cycle. The aim of this study was to examine the applicability of 2D speckle tracking echocardiography for comprehensive global and regional assessment of diastolic function in a pre-clinical murine model of cardio-metabolic disease. Type 2 diabetes (T2D) was induced in C57Bl/6 male mice using a high fat high sugar dietary intervention for 20 weeks. Significant impairment in left ventricle peak diastolic strain rate was evident in longitudinal, radial and circumferential planes in T2D mice. Peak diastolic velocity was similarly impaired in the longitudinal and radial planes. Regional analysis of longitudinal peak diastolic strain rate revealed that the anterior free left ventricular wall is particularly susceptible to T2D-induced diastolic dysfunction. These findings provide a significant advance on characterization of diastolic dysfunction in a pre-clinical mouse model of cardiopathology and offer a comprehensive suite of benchmark values for future pre-clinical cardiology studies.
舒张功能障碍越来越被认为是一种关键的、早期出现的亚临床状态，是包括糖尿病性心脏病和射血分数保留心力衰竭（HFpEF）在内的患病率不断上升的心脏病理的特征。舒张功能障碍的特征在疾病结果的管理中具有重要的预后价值。需要有经过验证的工具来监测糖尿病啮齿动物模型中的舒张功能，以便在临床前心脏病学研究中取得进展。二维斑点追踪超声心动图已成为评估整个心动周期心脏壁变形的有力工具。本研究旨在探讨二维斑点追踪超声心动图在评估心脏代谢疾病的临床前小鼠模型的全面的整体和区域性舒张功能中的适用性。使用高脂肪高糖饮食干预 20 周，在 C57Bl/6 雄性小鼠中诱导 2 型糖尿病（T2D）。在 T2D 小鼠中，纵向、径向和周向平面的左心室峰值舒张应变率明显受损。纵向和径向平面的峰值舒张速度也同样受损。纵向峰值舒张应变率的区域分析显示，前游离左心室壁特别容易受到 T2D 引起的舒张功能障碍的影响。这些发现为心脏病理的临床前小鼠模型中的舒张功能障碍的特征描述提供了重要进展，并为未来的临床前心脏病学研究提供了一套全面的基准值。
