Department of Biological Engineering, University of Idaho, Moscow, ID 83844, United States.
Department of Chemical Engineering, Michigan Technological University, Houghton, MI 49931, United States.
Bioresour Technol. 2018 May;256:548-551. doi: 10.1016/j.biortech.2018.02.074. Epub 2018 Feb 20.
In this study, an economical two-stage method was proposed for the production of natural astaxanthin from Haematococcus pluvialis without a medium replacement step. In stage 1, H. pluvialis were grown under low light illumination until they reached optimal biomass. In stage 2, cells were switched to astaxanthin induction conditions utilizing the combination of high light illumination and elevated carbon dioxide levels (5 or 15%). The introduction of CO altered the C/N balance creating a nutrient deficiency without a change of media. The resulting astaxanthin yield was 2-3 times that of using either stressor alone. This astaxanthin induction method has many advantages over current methods including no medium replacement and a short induction time of less than four days.
在这项研究中，提出了一种经济的两阶段方法，用于从雨生红球藻生产天然虾青素，而无需更换培养基步骤。在第一阶段，在低光照下培养雨生红球藻，直到达到最佳生物量。在第二阶段，利用高光照射和升高的二氧化碳水平（5%或 15%）将细胞切换到虾青素诱导条件。CO 的引入改变了 C/N 平衡，在不改变培养基的情况下造成营养缺乏。所得虾青素的产量是单独使用任一胁迫因素的 2-3 倍。与目前的方法相比，这种虾青素诱导方法具有许多优点，包括无需更换培养基和不到四天的短诱导时间。
