Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
Bioresour Technol. 2020 Mar;300:122594. doi: 10.1016/j.biortech.2019.122594. Epub 2019 Dec 13.
The optimization of the BG-11 culture medium nutrients using complex statistical design followed by incremental illumination was planned to stimulate the Haematococcus pluvialis growth and astaxanthin accumulation. Based on the Plackett-Burman design results, MgSO·7HO, HBO, and NaCO were identified as critical components to improve the biomass and astaxanthin productivity. Using central composite design, their initial critical concentrations in the green stage were found as 57.5, 6.2 and 53.0 mg/L, while for the red stage, the 138.3, 8.5 and 41.0 mg/L, recorded as optimum respectively. Using the optimum media, growth and astaxanthin accumulation at the end of the phototrophic and photoinduction stages were boosted by 17 and 54% respectively. The results of scale-up coupled with incremental illumination in phototrophic stage revealed the biomass and astaxanthin concentration improved 50% and 60% over the BG-11 media under constant light intensity. Also, different optimum culture medium formula for green and red stages was proposed.
采用复杂统计设计和逐步光照优化 BG-11 培养基的营养成分，以刺激雨生红球藻的生长和虾青素的积累。根据 Plackett-Burman 设计结果，确定 MgSO·7HO、HBO 和 NaCO 为提高生物量和虾青素生产力的关键成分。利用中心复合设计，发现其在绿藻阶段的初始关键浓度分别为 57.5、6.2 和 53.0mg/L，而在红藻阶段则分别为 138.3、8.5 和 41.0mg/L，分别为最佳值。在最佳培养基中，在光养和光诱导阶段结束时，生长和虾青素积累分别提高了 17%和 54%。在光养阶段逐步增加光照的放大效果表明，与恒定光强下的 BG-11 培养基相比，生物量和虾青素浓度提高了 50%和 60%。此外，还提出了绿藻和红藻阶段的不同最佳培养基配方。
