State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
Bioresour Technol. 2017 Nov;244(Pt 2):1439-1444. doi: 10.1016/j.biortech.2017.04.116. Epub 2017 May 4.
In vivo spatiotemporal dynamics of lipids and astaxanthin evolution in Haematococcus pluvialis mutant induced with 15% CO and high light intensity were monitored with high spatial resolution in a non-destructive and label-free manner using single-cell Raman imaging. Astaxanthin intensity increased by 3.5 times within 12h under 15% CO, and the accumulation rate was 5.8 times higher than that under air. Lipids intensity under 15% CO was 27% higher than that under air. The lipids initially concentrated in chloroplast under 15% CO due to an increase of directly photosynthetic fatty acid, which was different from the whole-cell dispersed lipids under air. Astaxanthin produced in chloroplast first accumulated around nucleus and then spread in cytoplasmic lipids under both air and 15% CO. The calculation results of kinetic models for lipids and astaxanthin evolutions showed that accumulation rate of lipids was much higher than that of astaxanthin in cells.
利用单细胞拉曼成像技术，以非破坏性和无标记的方式，以高空间分辨率监测了 15% CO 和高光强诱导的雨生红球藻突变体中脂质和虾青素的体内时空动态。在 15% CO 下，虾青素的浓度在 12 小时内增加了 3.5 倍，积累速度比空气下高 5.8 倍。15% CO 下的脂质强度比空气下高 27%。由于直接光合作用脂肪酸的增加，15% CO 下的脂质最初集中在叶绿体中，这与空气下全细胞分散的脂质不同。虾青素首先在叶绿体中产生，然后在空气和 15% CO 下在细胞质脂质中扩散。脂质和虾青素演化的动力学模型的计算结果表明，细胞中脂质的积累速度远高于虾青素。
