Melø T B, Reisaeter G
Z Naturforsch C J Biosci. 1986 Sep-Oct;41(9-10):867-72.
During growth of Propionibacterium acnes on Eagles medium protoporphyrin was accumulated inside the cells and coproporphyrin, both as a free base as metalcontaining, outside the cells. The photochemical processes in the endogenous porphyrins were studied by fluorescence spectroscopy during continuous irradiation of Propionibacterium acnes in suspension. The irradiation caused initially an increase in the content of protoporphyrin in the cells in comparison to that which had been accumulated during growth. Maximum light induced protoporphyrin production was achieved in 5 days old cultures. In old cultures where there was practically no initial protoporphyrin release, the fluorescence intensities from all the porphyrins present in the culture vanished exponentially with the irradiation time. The metal containing form of fluorescent coproporphyrin, with a maximum emission at 580 nm, was photobleached about ten times faster than the free base forms of coproporphyrin and protoporphyrin. Among these three fluorescent substances in the cell culture only the free base forms of the porphyrins have longer lifetimes than the cells themselves irradiated at the same conditions.
在痤疮丙酸杆菌在伊格尔培养基上生长期间，原卟啉在细胞内积累，而粪卟啉则以游离碱形式和含金属形式在细胞外积累。在悬浮培养的痤疮丙酸杆菌持续照射过程中，通过荧光光谱法研究了内源性卟啉中的光化学过程。与生长期间积累的相比，照射最初导致细胞中原卟啉含量增加。在5日龄培养物中实现了最大光诱导原卟啉产生。在几乎没有初始原卟啉释放的老龄培养物中，培养物中存在的所有卟啉的荧光强度随照射时间呈指数下降。荧光粪卟啉的含金属形式在580nm处有最大发射，其光漂白速度比粪卟啉和原卟啉的游离碱形式快约十倍。在细胞培养物中的这三种荧光物质中，只有卟啉的游离碱形式的寿命比在相同条件下照射的细胞本身更长。
