Kasuya Shin, Kato-Kogoe Nahoko, Omori Michi, Yamamoto Kayoko, Taguchi Shogo, Fujita Hiroshi, Imagawa Naoko, Sunano Akihiro, Inoue Kazuya, Ito Yuichi, Hirata Azumi, Ueno Takaaki, Moy Peter K
Fellow, Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organs, Faculty of Medicine, Osaka Medical College, Takatsuki, Japan.
Assistant Professor, Department of Dentistry and Oral Surgery, Division of Medicine for Function and Morphology of Sensor Organs, Faculty of Medicine, Osaka Medical College, Takatsuki, Japan.
Implant Dent. 2018 Apr;27(2):158-164. doi: 10.1097/ID.0000000000000738.
We carried out guided bone regeneration of cranial bone defects in rats using the bovine bone substitute Bio-Oss and a collagen membrane and performed histological observations of the bone repair process.
Bone defects were created in the cranial bones of 30 15-week-old Sprague-Dawley rats. We made 3 groups. A is unfilled, B is Bio-Oss, and C is Bio-Oss plus a collagen membrane. At 4 or 8 weeks postoperatively, tissue samples were taken. The Kawamoto technique was used for histological evaluation.
There was no new bone formation in group A. In groups B and C, new bone formation was evident around the Bio-Oss. In group C, new bone formation was evident in the centers of the bone defects, detached from the cut edge of the cranial bone.
Our results suggested that the Bio-Oss acts as a scaffold for bone repair, and the use of a collagen membrane may anchor the Bio-Oss closely to the cranial bone and assist the bone repair response.
我们使用牛骨替代物Bio-Oss和胶原膜对大鼠颅骨缺损进行引导性骨再生，并对骨修复过程进行组织学观察。
在30只15周龄的Sprague-Dawley大鼠的颅骨上制造骨缺损。我们分为3组。A组为未填充组，B组为Bio-Oss组，C组为Bio-Oss加胶原膜组。术后4周或8周时，采集组织样本。采用川本技术进行组织学评估。
A组没有新骨形成。在B组和C组中，Bio-Oss周围有明显的新骨形成。在C组中，骨缺损中心有明显的新骨形成，与颅骨切缘分离。
我们的结果表明，Bio-Oss作为骨修复的支架，使用胶原膜可能会使Bio-Oss紧密地附着在颅骨上，并协助骨修复反应。
