Santos Matheus Afm, Silva Daniela N, Rovaris Karla, Sousa Frederico B, Dantas Eugenia LA, Loureiro Lucas A, Pereira Thiago M C, Meyrelles Silvana S, Bertollo Rossiene M, Vasquez Elisardo C
Dentistry Graduate Program, Federal University of Espirito Santo, UFES, Vitoria, Brazil.
Department of Pathology & Clinical Dentistry, Federal University of Piaui, Teresina, Brazil.
Front Physiol. 2022 Feb 25;13:841146. doi: 10.3389/fphys.2022.841146. eCollection 2022.
Body bones play diverse pivotal roles, including the protection of vital organs. For instance, the integrative functions of the brain controlling diverse peripheral actions can be affected by a traumatic injury on the calvaria and the reparative process of a large defect is a challenge in the integrative physiology. Therefore, the development of biomaterials and approaches to improve such defects still requires substantial advances. In this regard, the most attractive approaches have been covering the cavity with inorganic bovine bone (IBB) and, more recently, also using low-level laser therapy (LT), but this issue has opened many questions. Here, it was determined the number of LT sessions required to speed up and to intensify the recovery process of two 5-mm-diameter defects promoted in the calvaria of each subgroup of six adult Wistar rats. The quantitative data showed that 30 days post-surgery, the recovery process by using blood clot-filling was not significantly influenced by the number of LT sessions. However, in the IBB-filled defects, the number of LT sessions markedly contributed to the improvement of the reparative process. Compared to the Control group (non-irradiated), the percentage of mineralization (formation of new bone into the cavities) gradually increased 25, 49, and 52% with, respectively, 4, 7, and 11 sessions of LT. In summary, combining the use of IBB with seven sessions of LT seems to be an optimal approach to greatly improve the recovery of calvarial defects. This translational research opens new avenues targeting better conditions of life for those suffering from large bone traumas and in the present field could contribute to preserve the integrative functions of the brain.
人体骨骼发挥着多种关键作用，包括保护重要器官。例如，控制各种外周动作的大脑整合功能可能会受到颅骨创伤的影响，而大骨缺损的修复过程对整合生理学来说是一项挑战。因此，生物材料的开发以及改善此类缺损的方法仍需取得重大进展。在这方面，最具吸引力的方法是用无机牛骨（IBB）覆盖骨腔，最近还采用了低强度激光疗法（LT），但这个问题引发了许多疑问。在此，确定了加速和强化六只成年Wistar大鼠各亚组颅骨上两个直径5毫米缺损恢复过程所需的LT疗程数。定量数据表明，术后30天，使用血凝块填充的恢复过程不受LT疗程数的显著影响。然而，在IBB填充的缺损中，LT疗程数对修复过程的改善有显著贡献。与对照组（未照射）相比，LT分别进行4、7和11次疗程时，矿化百分比（在骨腔内形成新骨）逐渐增加25%、49%和52%。总之，将IBB与七次LT疗程结合使用似乎是极大改善颅骨缺损恢复的最佳方法。这项转化研究为那些遭受严重骨创伤的人开创了新途径，以实现更好的生活条件，并且在当前领域有助于维持大脑的整合功能。
