Paknejad Mojgan, Rokn Amir Reza, Yaghobee Siamak, Moradinejad Pantea, Heidari Mohadeseh, Mehrfard Ali
Associate Professor, Dental Research Center Dentistry Research Institute Tehran University of Medical Sciences, Department of Periodontics,, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Professor, Member of Dental Implant Research Center of Tehran University of Medical Sciences, Department of Periodontics, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
J Dent (Tehran). 2014 Nov;11(6):687-95. Epub 2014 Nov 30.
The purpose of this study was to evaluate the efficacy of two types of bone substitutes, Bio-Oss and NuOss, for repair of bone defects.
This study was performed on the calvaria of 14 New Zealand rabbits. The 6mm critical size defect (CSD) models of bone regeneration were used. Three CSDs were created in each surgical site. The first defect was filled with NuOss, the second one with Bio-Oss and the third one remained unfilled as the control. After healing periods of one and two months (seven animal for each time point), histological and histomorphometric analyses were carried out to assess the amount of new bone formation, presence of inflammation, foreign body reaction and type of new bone. Qualitative variables were analyzed by multiple comparisons, Wilcoxon, Friedman and Mann Whitney tests. Quantitative variables were analyzed using the Mann-Whitney and Wilcoxon tests. Level of statistical significance was set at 0.05.
The level of inflammation was not significantly different at four and eight weeks in the Bio-Oss (P=0.944), NuOss (P=1.000) and control groups (P=0.71). At four weeks, foreign body reaction was not observed in Bio-Oss, NuOss and control groups. There was no significant difference in the type of the newly formed bone at four and eight weeks in any group (P=0.141 for Bio-Oss, P=0.06 for NuOss and P=0.389 for the control group).
Deproteinized bovine bone mineral can be used as a scaffold in bone defects to induce bone regeneration.
本研究旨在评估两种骨替代物Bio - Oss和NuOss修复骨缺损的疗效。
本研究在14只新西兰兔的颅骨上进行。采用6mm临界尺寸骨缺损（CSD）模型进行骨再生研究。每个手术部位制造3个CSD。第一个缺损填充NuOss，第二个填充Bio - Oss，第三个不填充作为对照。在愈合1个月和2个月后（每个时间点7只动物），进行组织学和组织形态计量学分析，以评估新骨形成量、炎症情况、异物反应及新骨类型。定性变量通过多重比较、Wilcoxon、Friedman和Mann Whitney检验进行分析。定量变量使用Mann - Whitney和Wilcoxon检验进行分析。统计学显著性水平设定为0.05。
Bio - Oss组（P = 0.944）、NuOss组（P = 1.000）和对照组（P = 0.71）在4周和8周时炎症水平无显著差异。在4周时，Bio - Oss组、NuOss组和对照组均未观察到异物反应。在任何组中，4周和8周时新形成骨的类型均无显著差异（Bio - Oss组P = 0.141，NuOss组P = 0.06，对照组P = 0.389）。
脱蛋白牛骨矿物质可作为骨缺损的支架材料诱导骨再生。
