Wirthgen Elisa, Goumon Sébastien, Kunze Martin, Walz Christina, Spitschak Marion, Tuchscherer Armin, Brown Jennifer, Höflich Christine, Faucitano Luigi, Hoeflich Andreas
Institute of Genome Biology, Leibniz-Institute for Farm Animal Biology (FBN), Dummerstorf, Germany.
Department of Ethology, Institute of Animal Science, Prague, Czechia.
Front Endocrinol (Lausanne). 2018 Feb 13;9:36. doi: 10.3389/fendo.2018.00036. eCollection 2018.
In previous work using market-weight pigs, we had demonstrated that insulin-like growth factors (IGFs) and insulin-like growth factor binding proteins (IGFBPs) are regulated during shipment characterized by changing conditions of stress due to loading or unloading, transportation, lairage, and slaughter. In addition, we found in a previous study that IGFBP-2 concentrations were lower in pigs transported for longer periods of time. Therefore, we performed a more detailed study on the effects of transport duration and season on the plasma concentrations of IGFs and IGFBPs in adult pigs. For the study, exsanguination blood was collected from 240 market-weight barrows that were transported for 6, 12, or 18 h in January or July. IGF-I and -II were detected using commercial ELISAs whereas IGFBPs were quantified by quantitative Western ligand blotting. In addition, established markers of stress and metabolism were studied in the animals. The results show that plasma concentrations of IGFBP-3 were significantly reduced after 18 h of transport compared to shorter transport durations (6 and 12 h; < 0.05). The concentrations of IGF-I in plasma were higher ( < 0.001) in pigs transported 12 h compared to shorter or longer durations. Season influenced plasma concentrations of IGFBP-3 and IGF-II ( < 0.05 and < 0.01, respectively). Neither transport duration nor differential environmental conditions of winter or summer had an effect on glucocorticoids, albumin, triglycerides, or glucose concentrations ( > 0.05). However, low-density lipoprotein concentrations decreased after 18 h compared to 6 h of transport ( < 0.05), whereas high-density lipoprotein concentrations were higher ( < 0.05) in pigs transported for 12 or 18 h compared to those transported for only 6 h. Our findings indicate differential regulation of IGF-compounds in response to longer transport duration or seasonal changes and support current evidence of IGFs and IGFBPs as innovative animal-based indicators of psycho-social or metabolic stress in pigs.
在之前使用市场体重猪的研究中，我们已经证明，在运输过程中，胰岛素样生长因子（IGFs）和胰岛素样生长因子结合蛋白（IGFBPs）会受到调节，运输过程的特征是由于装卸、运输、畜栏饲养和屠宰等导致应激条件不断变化。此外，我们在之前的一项研究中发现，运输时间较长的猪体内IGFBP - 2浓度较低。因此，我们针对运输时长和季节对成年猪血浆中IGFs和IGFBPs浓度的影响进行了更详细的研究。在这项研究中，从240头市场体重的公猪采集放血后的血液，这些公猪于1月或7月分别运输6、12或18小时。使用商用酶联免疫吸附测定法（ELISA）检测IGF - I和 - II，而通过定量western配体印迹法定量IGFBPs。此外，还研究了这些动物体内已确定的应激和代谢标志物。结果显示，与较短运输时长（6小时和12小时）相比，运输18小时后血浆中IGFBP - 3浓度显著降低（P < 0.05）。与较短或较长运输时长相比，运输12小时的猪血浆中IGF - I浓度更高（P < 0.001）。季节影响血浆中IGFBP - 3和IGF - II的浓度（分别为P < 0.05和P < 0.01）。运输时长和冬季或夏季不同的环境条件均未对糖皮质激素、白蛋白、甘油三酯或葡萄糖浓度产生影响（P > 0.05）。然而，与运输6小时相比，运输18小时后低密度脂蛋白浓度降低（P < 0.05），而运输12或18小时的猪与仅运输6小时的猪相比，高密度脂蛋白浓度更高（P < 0.05）。我们的研究结果表明，IGF复合物会因运输时长延长或季节变化而受到不同调节，并支持目前将IGFs和IGFBPs作为猪心理 - 社会或代谢应激创新性动物指标的证据。
