Lavasani Paria Sadat, Motevaseli Elahe, Shirzad Mahdieh, Modarressi Mohammad Hossein
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Microbiology, School of Biology, College of Sciences, Tehran University, Tehran, Iran.
Iran J Microbiol. 2017 Dec;9(6):338-347.
Acetic acid bacteria (AAB) are one of the major interests of researchers. Traditional vinegars are suitable sources of AAB because they are not undergone industrial process like filtering and adding preservatives. as a member of AAB is known as the main cellulose producer among other bacteria. The purpose of the current study was to isolate the bacteria from traditional vinegars and its molecular analyses.
Vinegar samples were collected. Well-organized bacteriological tests were carried out to differentiate isolated bacteria from other cellulose producers and to identify . NaOH treatment and Calcofluor white staining were used for detecting cellulose. Chromosomal DNA of each strain was extracted via three methods of boiling, phenol-chloroform and sonication. Molecular analyses were performed on the basis of 16S rRNA sequences and cellulose synthase catalytic subunit gene for further confirmation. Phylogenetic tree was constructed for more characterization. Two housekeeping genes were studied including phenylalanyl-tRNA synthase alpha subunit and RNA polymerase alpha subunit .
Of the 97 samples, 43 strains were isolated. They were identified via bacteriological and molecular techniques. 16S rDNA sequence showed 99% similarity with registered sequences of the bacteria. Biodiversity of the genome confirmed by analyzing and genes.
can be isolated from traditional vinegars. Screening tests ought to include the classical methods and molecular techniques. Different molecular techniques and more genomic research should be developed to expand our knowledge for distinguishing isolated bacteria especially in the fields of AAB.
醋酸菌是研究人员主要关注的对象之一。传统醋是醋酸菌的合适来源，因为它们未经过如过滤和添加防腐剂等工业加工过程。作为醋酸菌的一员，某菌在其他细菌中是主要的纤维素生产者。本研究的目的是从传统醋中分离细菌并进行分子分析。
收集醋样。进行了系统的细菌学测试，以区分分离出的细菌与其他纤维素生产者，并进行鉴定。采用氢氧化钠处理和荧光增白剂染色检测纤维素。通过煮沸、酚 - 氯仿和超声处理三种方法提取各菌株的染色体DNA。基于16S rRNA序列和纤维素合酶催化亚基基因进行分子分析以进一步确认。构建系统发育树以进行更多特征描述。研究了两个管家基因，包括苯丙氨酰 - tRNA合成酶α亚基和RNA聚合酶α亚基。
在97个样本中，分离出43株菌株。通过细菌学和分子技术对它们进行了鉴定。16S rDNA序列与已登记的细菌序列显示出99%的相似性。通过分析某基因和某基因确认了基因组的生物多样性。
可从传统醋中分离出某菌。筛选试验应包括经典方法和分子技术。应开发不同的分子技术和更多的基因组研究，以扩展我们区分分离细菌的知识，特别是在醋酸菌领域。
