Küçükkaya Eren Selen, Uzunoğlu Emel, Sezer Banu, Yılmaz Zeliha, Boyacı İsmail Hakkı
Department of Endodontics, Faculty of Dentistry, Hacettepe University, Ankara, Turkey.
Department of Food Engineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.
Restor Dent Endod. 2018 Feb 4;43(1):e11. doi: 10.5395/rde.2018.43.e11. eCollection 2018 Feb.
This study aimed to introduce the use of laser-induced breakdown spectroscopy (LIBS) for evaluation of the mineral content of root canal dentin, and to assess whether a correlation exists between LIBS and scanning electron microscopy/energy dispersive spectroscopy (SEM/EDS) methods by comparing the effects of irrigation solutions on the mineral content change of root canal dentin.
Forty teeth with a single root canal were decoronated and longitudinally sectioned to expose the canals. The root halves were divided into 4 groups ( = 10) according to the solution applied: group NaOCl, 5.25% sodium hypochlorite (NaOCl) for 1 hour; group EDTA, 17% ethylenediaminetetraacetic acid (EDTA) for 2 minutes; group NaOCl+EDTA, 5.25% NaOCl for 1 hour and 17% EDTA for 2 minutes; a control group. Each root half belonging to the same root was evaluated for mineral content with either LIBS or SEM/EDS methods. The data were analyzed statistically.
In groups NaOCl and NaOCl+EDTA, the calcium (Ca)/phosphorus (P) ratio decreased while the sodium (Na) level increased compared with the other groups ( < 0.05). The magnesium (Mg) level changes were not significant among the groups. A significant positive correlation was found between the results of LIBS and SEM/EDS analyses ( = 0.84, < 0.001).
Treatment with NaOCl for 1 hour altered the mineral content of dentin, while EDTA application for 2 minutes had no effect on the elemental composition. The LIBS method proved to be reliable while providing data for the elemental composition of root canal dentin.
本研究旨在介绍激光诱导击穿光谱法（LIBS）用于评估根管牙本质矿物质含量的应用，并通过比较冲洗液对根管牙本质矿物质含量变化的影响，评估LIBS与扫描电子显微镜/能谱仪（SEM/EDS）方法之间是否存在相关性。
选取40颗单根管牙，去除牙冠并纵向剖开以暴露根管。根据所应用的溶液将牙根半段分为4组（每组n = 10）：次氯酸钠组，5.25%次氯酸钠（NaOCl）处理1小时；乙二胺四乙酸组，17%乙二胺四乙酸（EDTA）处理2分钟；次氯酸钠+乙二胺四乙酸组，5.25% NaOCl处理1小时，17% EDTA处理2分钟；对照组。同一牙根的每个牙根半段采用LIBS或SEM/EDS方法评估矿物质含量。对数据进行统计学分析。
与其他组相比，次氯酸钠组和次氯酸钠+乙二胺四乙酸组中钙（Ca）/磷（P）比值降低，而钠（Na）水平升高（P < 0.05）。各组间镁（Mg）水平变化不显著。LIBS和SEM/EDS分析结果之间存在显著正相关（r = 0.84，P < 0.001）。
用NaOCl处理1小时会改变牙本质的矿物质含量，而应用EDTA 2分钟对元素组成无影响。LIBS方法在为根管牙本质元素组成提供数据时被证明是可靠的。
