University of Illinois at Urbana-Champaign, Beckman Institute of Advanced Science and Technology, De, United States.
Fujian Normal University, Key Laboratory of OptoElectronic Science and Technology for Medicine of Mi, China.
J Biomed Opt. 2018 Feb;23(2):1-6. doi: 10.1117/1.JBO.23.2.025003.
Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide a valuable alternative for this type of assay. We employ spatial light interference microscopy (SLIM) to perform high-accuracy single-cell phase imaging and decouple the average thickness and refractive index information for the population. This procedure was enabled by quantitative-phase imaging cells on media of two different refractive indices and using a numerical tool to remove the curvature from the cell tails. This way, we achieved ensemble averaging of topography and refractometry of 100 cells in each of the two groups. The results show that the thickness profile of the cell tail goes down to 150 nm and the refractive index can reach values of 1.6 close to the head.
精子活力的特征是治疗不孕不育的常用测试。最近，无标记、相敏成像已被证明是此类检测的一种很有价值的替代方法。我们采用空间光干涉显微镜（SLIM）进行高精度的单细胞相成像，并解耦了群体的平均厚度和折射率信息。通过在两种不同折射率的介质上对细胞进行定量相成像，并使用数值工具去除细胞尾部的曲率，从而实现了这一过程。通过这种方式，我们在每组中对 100 个细胞的形貌和折射率进行了整体平均。结果表明，细胞尾部的厚度轮廓下降到 150nm，折射率可以达到接近头部的 1.6 值。
