Omniome, Inc. , 10575 Roselle Street , San Diego , California 92121 , United States.
ACS Sens. 2018 Mar 23;3(3):561-568. doi: 10.1021/acssensors.7b00957. Epub 2018 Mar 2.
We demonstrated a proof-of-principle concept of a label-free platform that enables nucleic acid sequencing by binding methodology. The system utilizes gold surfaces having high fidelity plasmonic nanohole arrays which are very sensitive to minute changes of local refractive indices. Our novel surface chemistry approach ensures accurate identification of correct bases at individual positions along a targeted DNA sequence on the gold surface. Binding of the correct base on the gold sensing surface triggers strong spectral variations within the nanohole optical response, which provides a high signal-to-noise ratio and accurate sequence data. Integrating our label-free sequencing platform with a lens-free imaging-based device, we reliably determined targeted DNA sequences by monitoring the changes within the plasmonic diffraction images. Consequently, this new label-free surface chemistry technique, integrated with plasmonic lens-free imaging platform, will enable monitoring multiple biomolecular binding events, which could initiate new avenues for high-throughput nucleic acid sequencing.
我们展示了一种基于结合方法的无标记平台的原理验证概念，该平台可实现核酸测序。该系统利用具有高保真度等离子纳米孔阵列的金表面，这些表面对局部折射率的微小变化非常敏感。我们新颖的表面化学方法可确保在金表面上的目标 DNA 序列的各个位置准确识别正确的碱基。在金感应表面上正确碱基的结合会引发纳米孔光学响应中的强光谱变化，从而提供高信噪比和准确的序列数据。通过将无标记测序平台与无透镜成像设备集成，我们通过监测等离子体衍射图像中的变化可靠地确定了目标 DNA 序列。因此，这种新的无标记表面化学技术与等离子体无透镜成像平台相结合，将能够监测多个生物分子结合事件，从而为高通量核酸测序开辟新途径。
