Departments of Epidemiology and Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA.
Departments of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University, Atlanta, GA.
J Nutr. 2018 Feb 1;148(2):245-253. doi: 10.1093/jn/nxx045.
Both systemic redox status and diet quality are associated with risk outcomes in chronic disease. It is not known, however, the extent to which diet quality influences plasma thiol/disulfide redox status.
The purpose of this study was to investigate the influence of diet, as measured by diet quality scores and other dietary factors, on systemic thiol/disulfide redox status.
We performed a cross-sectional study of 685 working men and women (ages ≥18 y) in Atlanta, GA. Diet was assessed by 3 diet quality scores: the Alternative Healthy Eating Index (AHEI), Dietary Approaches to Stop Hypertension (DASH), and the Mediterranean Diet Score (MDS). We measured concentrations of plasma glutathione (GSH), cysteine, their associated oxidized forms [glutathione disulfide (GSSG) and cystine (CySS), respectively], and their redox potentials (EhGSSG and EhCySS) to determine thiol/disulfide redox status. Linear regression modeling was performed to assess relations between diet and plasma redox after adjustment for age, body mass index (BMI), sex, race, and history of chronic disease.
MDS was positively associated with plasma GSH (β = 0.02; 95% CI: 0.003, 0.03) and total GSH (GSH + GSSG) (β = 0.02; 95% CI: 0.003, 0.03), and inversely associated with the CySS:GSH ratio (β = -0.02; 95% CI: -0.04, -0.004). There were significant independent associations between individual MDS components (dairy, vegetables, fish, and monounsaturated fat intake) and varying plasma redox indexes (P < 0.05). AHEI and DASH diet quality indexes and other diet factors of interest were not significantly correlated with plasma thiol and disulfide redox measures.
Adherence to the Mediterranean diet was significantly associated with a favorable plasma thiol/disulfide redox profile, independent of BMI, in a generally healthy working adult population. Although longitudinal studies are warranted, these findings contribute to the feasibility of targeting a Mediterranean diet to improve plasma redox status.
全身氧化还原状态和饮食质量都与慢性病的风险结果有关。然而，饮食质量对血浆硫醇/二硫键氧化还原状态的影响程度尚不清楚。
本研究旨在调查饮食（通过饮食质量评分和其他饮食因素来衡量）对全身硫醇/二硫键氧化还原状态的影响。
我们对佐治亚州亚特兰大的 685 名在职男性和女性（年龄≥18 岁）进行了横断面研究。饮食质量通过 3 种饮食质量评分进行评估：替代健康饮食指数（AHEI）、停止高血压的饮食方法（DASH）和地中海饮食评分（MDS）。我们测量了血浆谷胱甘肽（GSH）、半胱氨酸及其相关氧化形式[谷胱甘肽二硫化物（GSSG）和胱氨酸（CySS）]的浓度，以确定硫醇/二硫键氧化还原状态。在调整年龄、体重指数（BMI）、性别、种族和慢性病史后，采用线性回归模型评估饮食与血浆氧化还原之间的关系。
MDS 与血浆 GSH（β=0.02；95%CI：0.003，0.03）和总 GSH（GSH+GSSG）（β=0.02；95%CI：0.003，0.03）呈正相关，与 CySS：GSH 比值（β=-0.02；95%CI：-0.04，-0.004）呈负相关。MDS 的个别成分（奶制品、蔬菜、鱼类和单不饱和脂肪摄入）与不同的血浆氧化还原指标之间存在显著的独立关联（P<0.05）。AHEI 和 DASH 饮食质量指数和其他感兴趣的饮食因素与血浆硫醇和二硫键氧化还原测量值无显著相关性。
在一般健康的成年人群中，地中海饮食的依从性与有利的血浆硫醇/二硫键氧化还原状态显著相关，与 BMI 无关。虽然需要进行纵向研究，但这些发现为靶向地中海饮食改善血浆氧化还原状态的可行性提供了依据。
