Sun Ting, Guo Mengmeng, Cheng Yaodong, Ou Lingling, He Ping, Liu Xiangning, Ma Shuyuan, Lai Renfa
J Biomed Nanotechnol. 2017 Dec 1;13(12):1682-1693. doi: 10.1166/jbn.2017.2379.
A graded glass/graded zirconia (G/Z) system was previously synthesized via the infiltration of a low modulus nanosized glass into a zirconia surface. The bond strength of G/Z to veneering porcelains was demonstrated to be 3-fold higher than in zirconia-based systems. Nevertheless, biocompatibility testing prior to the possible clinical application of G/Z systems is essential. Herein, such biocompatibility testing was performed with L-929 fibroblasts seeded onto G/Z and yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) for 2-72 h. Assessments included an oral mucous membrane irritation test in conjunction with analyses of cell viability, cell morphology, cell cycle, cell apoptosis, oxidative stress responses, inflammatory cytokine expression, and cellular toxicity. Cell viability tests showed no significant decrease in G/Z- and Y-TZP-treated cells over 72 h. Fluorescence and SEM images demonstrated that cell spreading on Y-TZP and G/Z was similar; cells were flattened and well-spread. Oxidative stress data for G/Z- and Y-TZP-treated cells showed no significant difference in ROS production. Cellular toxicity results for G/Z did not elicit significant differences in LDH release compared with Y-TZP over 72 h. G/Z and Y-TZP had no significant differences in IL-1α, IL-8, PGE2, and TNF-α expression and elicited significantly increased IL-8 expression compared with that of the untreated control group. Cells that were cultured with G/Z showed no significant changes in cell cycle distribution compared with Y-TZP or the untreated control group. Cells that were cultured with Y-TZP and G/Z showed no apoptosis compared to untreated controls at 24 and 48 h. According to the oral mucous membrane irritation test, scores for the macroscopic and microscopic observations for both G/Z and Y-TZP sides were 0, demonstrating no consequent irritation. Therefore, the excellent biocompatibility of G/Z indicates that it has potential for future clinical applications.
一种梯度玻璃/梯度氧化锆（G/Z）系统先前是通过将低模量纳米级玻璃渗入氧化锆表面而合成的。结果表明，G/Z与饰面瓷的结合强度比基于氧化锆的系统高3倍。然而，在G/Z系统可能用于临床之前进行生物相容性测试至关重要。在此，将L-929成纤维细胞接种到G/Z和钇稳定四方氧化锆多晶体（Y-TZP）上2至72小时，进行了这种生物相容性测试。评估包括口腔黏膜刺激试验以及细胞活力、细胞形态、细胞周期、细胞凋亡、氧化应激反应、炎性细胞因子表达和细胞毒性分析。细胞活力测试表明，在72小时内，G/Z和Y-TZP处理的细胞没有显著减少。荧光和扫描电子显微镜图像显示，细胞在Y-TZP和G/Z上的铺展情况相似；细胞扁平且铺展良好。G/Z和Y-TZP处理的细胞的氧化应激数据显示，活性氧产生没有显著差异。与Y-TZP相比，G/Z的细胞毒性结果在72小时内乳酸脱氢酶释放没有显著差异。G/Z和Y-TZP在白细胞介素-1α、白细胞介素-8、前列腺素E2和肿瘤坏死因子-α表达方面没有显著差异，与未处理的对照组相比，白细胞介素-8表达显著增加。与Y-TZP或未处理的对照组相比，用G/Z培养的细胞在细胞周期分布上没有显著变化。与未处理的对照组相比，在24小时和48小时时，用Y-TZP和G/Z培养的细胞没有凋亡。根据口腔黏膜刺激试验，G/Z和Y-TZP两侧的宏观和微观观察评分均为0，表明没有后续刺激。因此，G/Z优异的生物相容性表明它在未来临床应用中具有潜力。
