Varghese Finny S, van Rij Ronald P
Department of Medical Microbiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
Methods Mol Biol. 2018;1746:197-213. doi: 10.1007/978-1-4939-7683-6_16.
Insects are the most abundant and diverse group of animals on earth, but our knowledge of their viruses is biased toward insect-borne viruses that cause disease in plants, animals, or humans. Recent metagenomic studies and systematic surveys of viruses in wild-caught insects have identified an unanticipated large repertoire of novel viruses and viral sequences. These include new members of existing clades, new clades, and even entirely new virus families. These studies greatly expand the known virosphere in insects, provide opportunities to study virus-host interactions, and generate new insights into virus evolution. In this chapter, we discuss the methods used to identify novel viruses in insects and highlight some notable surprises arising from these studies.
昆虫是地球上数量最多、种类最丰富的动物群体，但我们对其病毒的了解主要集中在那些能在植物、动物或人类中引发疾病的虫媒病毒上。最近针对野外捕获昆虫开展的宏基因组学研究以及病毒系统调查，发现了数量惊人的新型病毒和病毒序列。这些包括现有进化枝的新成员、新的进化枝，甚至全新的病毒科。这些研究极大地扩展了昆虫中已知的病毒圈，为研究病毒与宿主的相互作用提供了机会，并为病毒进化带来了新的见解。在本章中，我们将讨论用于识别昆虫中新型病毒的方法，并着重介绍这些研究带来的一些显著惊喜。
