Britt Kellee, Gebben Samantha, Levy Amit, Achor Diann, Sieburth Peggy, Stevens Kristian, Al Rwahnih Maher, Batuman Ozgur
Department of Plant Pathology, Southwest Florida Research and Education Center, University of Florida, Immokalee, FL 34142, USA.
Department of Plant Pathology, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA.
Insects. 2022 Mar 10;13(3):275. doi: 10.3390/insects13030275.
The destructive citrus disease, Huanglongbing (HLB) or citrus greening, continues to devastate Florida's citrus industry. A hemipteran insect, the Asian citrus psyllid (ACP), disperses Liberibacter asiaticus, one of the putative bacterial pathogens of HLB. This study builds upon ongoing research utilizing high-throughput sequencing to analyze the virome of ACP populations collected from citrus groves throughout Florida. Following the widespread detection of sequences aligning to the genome of citrus tristeza virus (CTV) across consecutive years in the Florida ACP virome, we continued to detect a pervasive amount of CTV in Florida ACPs during subsequent years. Simultaneously, we also detected mixed infections of CTV strains in pooled ACPs from different Florida regions. Predating the HLB epidemic, CTV has been present in Florida for many years and our results confirm its widespread and diverse persistence in Florida citrus groves through a unique lens, the ACP. CTV presence in the ACP likely results from feeding on CTV-infected citrus trees in Florida citrus groves, which may help to understand an overlapping presence of CTV and HLB, both endemic citrus pathosystems in the state, and their role in future integrated pest management strategies.
具有破坏性的柑橘病害——黄龙病（HLB）或柑橘绿变病，持续重创佛罗里达州的柑橘产业。半翅目昆虫亚洲柑橘木虱（ACP）传播亚洲韧皮杆菌，这是HLB的一种假定细菌病原体。本研究基于正在进行的利用高通量测序分析从佛罗里达州各地柑橘园采集的ACP种群病毒组的研究。在佛罗里达州ACP病毒组中连续多年广泛检测到与柑橘衰退病毒（CTV）基因组比对的序列之后，在随后几年里我们继续在佛罗里达州的ACP中检测到大量普遍存在的CTV。同时，我们还在来自佛罗里达州不同地区的混合ACP中检测到CTV毒株的混合感染。在HLB流行之前，CTV已在佛罗里达州存在多年，我们的结果通过一个独特视角——ACP，证实了它在佛罗里达州柑橘园广泛且多样的持续存在。ACP中存在CTV可能是因为取食了佛罗里达州柑橘园里受CTV感染的柑橘树，这可能有助于理解CTV和HLB（该州两种地方性柑橘病害系统）的重叠存在情况以及它们在未来综合虫害管理策略中的作用。
