Park Hye Jung, Oh Eun-Yi, Park Yoon Hee, Yang Misuk, Park Kyung Hee, Park Jung-Won, Lee Jae-Hyun
a Department of Internal Medicine , Gangnam Severance Hospital, Yonsei University College of Medicine , Seoul , Korea.
b Institute of Allergy , Yonsei University College of Medicine , Seoul , Korea.
Biomarkers. 2018 Jul;23(5):446-452. doi: 10.1080/1354750X.2018.1443510. Epub 2018 Apr 12.
CD93 is a membrane-associated glycoprotein, which can be released in a soluble form (sCD93) into the serum. CD93 has received renewed attention as a candidate biomarker of inflammation in various inflammatory and immune-mediated diseases, including asthma.
We aimed to evaluate the effects of airway inflammation on CD93 levels in murine models.
We established an ovalbumin (OVA)-induced acute asthma murine model (OVA model) and a lipopolysaccharide (LPS)-induced airway inflammation murine model (LPS model). Dexamethasone was administered by gavage to attenuate the airway inflammation.
The OVA model demonstrated typical allergic asthma features with increased airway hyper-responsiveness, inflammatory cell infiltration, increased Th2 cytokine levels, compared to the control group. CD93 levels were decreased in lung homogenates and, respiratory epithelial cells, whereas serum sCD93 levels were increased in the OVA model, as compared to the control group. Dexamethasone reversed these effects of OVA. In contrast, in the LPS model, CD93 levels were not affected in neither respiratory epithelial cells nor serum.
Our findings demonstrate the potential of using sCD93 as a biomarker for allergic asthma.
CD93是一种膜相关糖蛋白，可呈可溶性形式（sCD93）释放到血清中。作为包括哮喘在内的各种炎症和免疫介导疾病中炎症的候选生物标志物，CD93再次受到关注。
我们旨在评估气道炎症对小鼠模型中CD93水平的影响。
我们建立了卵清蛋白（OVA）诱导的急性哮喘小鼠模型（OVA模型）和脂多糖（LPS）诱导的气道炎症小鼠模型（LPS模型）。通过灌胃给予地塞米松以减轻气道炎症。
与对照组相比，OVA模型表现出典型的过敏性哮喘特征，气道高反应性增加、炎症细胞浸润、Th2细胞因子水平升高。与对照组相比，OVA模型中肺匀浆和呼吸道上皮细胞中的CD93水平降低，而血清sCD93水平升高。地塞米松逆转了OVA的这些作用。相比之下，在LPS模型中，呼吸道上皮细胞和血清中的CD93水平均未受到影响。
我们的研究结果证明了使用sCD93作为过敏性哮喘生物标志物的潜力。
