Department of Internal Medicine, College of Medicine, Korea University, Seoul 02841, Korea.
Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, Korea University, Seoul 02841, Korea.
Int J Mol Sci. 2021 Dec 24;23(1):181. doi: 10.3390/ijms23010181.
Recent studies on the pathophysiology of irritable bowel syndrome (IBS) have focused on the role of mast cells (MCs) in intestinal mucosal immunity. A link between allergic airway diseases (AADs) and IBS has been suggested because both diseases have similar pathophysiology. We aimed to investigate whether the induction of AAD in mice could lead to inflammation of the colonic mucosa, similar to IBS. We also evaluated whether this inflammatory response could be suppressed by administering a therapeutic agent. Mice were divided into three groups: control, AAD-induced, and salbutamol-treated. An AAD mouse model was established by intraperitoneal injection and nasal challenge with ovalbumin. Mice with AAD were intranasally administered salbutamol. Analyses of cytokine levels, MC count, and tryptase levels in the intestinal mucosa were performed to compare the changes in inflammatory responses among the three groups. Inflammation was observed in the intestinal mucosa of mice in the AAD group. This inflammation in AAD mice was suppressed after salbutamol treatment. Our study demonstrates that AAD induces an inflammatory response similar to that in IBS, suggesting a possible association between IBS and AADs. In patients with IBS with such allergic components, salbutamol may have the potential to alleviate the inflammatory response.
最近对肠易激综合征（IBS）病理生理学的研究集中在肥大细胞（MCs）在肠道黏膜免疫中的作用上。由于这两种疾病具有相似的病理生理学，因此有人提出过敏性气道疾病（AAD）和 IBS 之间存在关联。我们旨在研究在小鼠中诱导 AAD 是否会导致类似于 IBS 的结肠黏膜炎症，并评估通过给予治疗剂是否可以抑制这种炎症反应。将小鼠分为三组：对照组、AAD 诱导组和沙丁胺醇治疗组。通过腹腔内注射和卵清蛋白鼻腔挑战建立 AAD 小鼠模型。对 AAD 小鼠进行沙丁胺醇鼻腔给药。分析细胞因子水平、肠黏膜中的 MC 计数和胰蛋白酶水平，以比较三组之间炎症反应的变化。在 AAD 组的小鼠的肠道黏膜中观察到炎症。沙丁胺醇治疗后，AAD 小鼠的这种炎症得到抑制。我们的研究表明，AAD 会引起类似于 IBS 的炎症反应，提示 IBS 和 AAD 之间可能存在关联。在具有此类过敏成分的 IBS 患者中，沙丁胺醇可能具有缓解炎症反应的潜力。
