National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Institute of Crop Sciences, Chinese Academy of Agriculture Sciences (CAAS), Beijing 100081, China.
Int J Mol Sci. 2018 Mar 2;19(3):717. doi: 10.3390/ijms19030717.
Bacterial blight, caused by pv. (), is an overwhelming disease in rice-growing regions worldwide. Our previous studies revealed that the executor gene confers broad-spectrum disease resistance to all naturally occurring biotypes of . In this study, comparative transcriptomic profiling of two near-isogenic lines (NILs), CBB23 (harboring ) and JG30 (without ), before and after infection of the strain, PXO99, was done by RNA sequencing, to identify genes associated with the resistance. After high throughput sequencing, 1645 differentially expressed genes (DEGs) were identified between CBB23 and JG30 at different time points. Gene Ontlogy (GO) analysis categorized the DEGs into biological process, molecular function, and cellular component. KEGG analysis categorized the DEGs into different pathways, and phenylpropanoid biosynthesis was the most prominent pathway, followed by biosynthesis of plant hormones, flavonoid biosynthesis, and glycolysis/gluconeogenesis. Further analysis led to the identification of differentially expressed transcription factors (TFs) and different kinase responsive genes in CBB23, than that in JG30. Besides TFs and kinase responsive genes, DEGs related to ethylene, jasmonic acid, and secondary metabolites were also identified in both genotypes after PXO99 infection. The data of DEGs are a precious resource for further clarifying the network of -mediated resistance.
细菌性条斑病由 pv. ()引起，是全球水稻种植区的一种毁灭性疾病。我们之前的研究表明，基因赋予了对所有自然发生的 pv. 生物型的广谱抗病性。在这项研究中，通过 RNA 测序对两个近等基因系（NIL）CBB23（携带）和 JG30（不携带）在感染 pv. PXO99 菌株前后进行了比较转录组分析，以鉴定与抗性相关的基因。在高通量测序后，在不同时间点，CBB23 和 JG30 之间鉴定出 1645 个差异表达基因（DEGs）。基因本体论（GO）分析将 DEGs 分为生物过程、分子功能和细胞成分。KEGG 分析将 DEGs 分为不同的途径，苯丙烷生物合成是最突出的途径，其次是植物激素生物合成、类黄酮生物合成和糖酵解/糖异生。进一步的分析导致鉴定出 CBB23 中差异表达的转录因子（TFs）和不同的激酶响应基因，而 JG30 中则没有。除了 TF 和激酶响应基因外，在 PXO99 感染后，两种基因型中还鉴定出与乙烯、茉莉酸和次生代谢物相关的 DEGs。DEGs 的数据是进一步阐明 pv. 介导的抗性网络的宝贵资源。
