AJCR4: A Potential Probiotic in the Fight Against Oral spp. Biofilms.

Oral candidiasis is one of the most common infections in the immunocompromised. Biofilms of species can make treatments difficult, leading to oral infection recurrence. This research aimed to isolate a with anti- effects from the oral cavity. An oral was isolated in caries-free individuals. The best isolate was evaluated against spp. planktonic and biofilm forms. The bacterial impacts on biofilms' adhesion to acrylic discs were analyzed through an in vitro test. AJCR4 had the best anti- activity in the preliminary screening. Results were promising in both planktonic and biofilms, particularly with SC5314 and ATCC750, where no viable cells were detected when using the cell-free supernatant (undiluted). In ATCC2001 and ATCC22019 biofilms, reductions of 3 Log and more than 2 Log, respectively, were noted when using a cell suspension of ACJR4 (10 CFU/mL). On polymethyl methacrylate acrylic discs, the cell-free supernatant reduced adhesion, resulting in no viable cell detection on the surface. In conclusion, AJCR4 demonstrated notable antifungal activity against biofilms. This oral isolate and its postbiotic can be a potential alternative strategy to oral candidiasis, especially to treat recalcitrant infections.