Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China.
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Biomaterials. 2018 May;165:39-47. doi: 10.1016/j.biomaterials.2018.02.042. Epub 2018 Feb 23.
Combination of biological entities with functional nanostructure would produce the excellent systemic drug-delivery vehicles that possess the ability to cross the biological barriers. Herein, from a biomimetic point of view, erythrocyte membrane bioinspired optical nanocarrier is fabricated by integrating Red blood cell (RBC) membrane vesicle with near-infrared persistent luminescence nanophosphors (PLNPs). The triple-doped zinc gallogermanate nanostructures with super-long near-infrared persistent luminescence (ZGGO) are used as optical emission center, mesoporous silica coated on the PLNPs (ZGGO@mSiO) is employed for drug delivery, and the RBC membrane vesicle is introduced for biomimetic functionalization to ensure the developed nanocarriers bypass macrophage uptake and systemic clearance. Owing to the coating of natural erythrocyte membrane along with membrane lipids and associated membrane proteins, the proposed bioinspired nanocarriers have exhibited cell-mimicking property. Retaining the applicability of PLNPs core that favored in vitro excitation, the developed RBC-ZGGO@mSiO biomimetic nanocarriers have demonstrated intense fluorescence, super-long persistent luminescence, monodispersed nanosize, red light renewability, and excellent biocompatibility. In vivo mice bioimaging and biodistribution study demonstrate the erythrocyte membrane bioinspired nanoprobe loaded with doxorubicin as ideal nanocarriers for long-circulating bioimaging, in situ real-time monitoring and drug delivery. We believe the PLNPs-based biomimetic nanocarriers offer a promising nano-platform for diagnostics and therapeutics application.
将生物实体与功能纳米结构相结合，将产生具有穿越生物屏障能力的优秀系统药物输送载体。在此，从仿生学的角度来看，通过将红细胞膜囊泡与近红外持续发光纳米荧光粉（PLNPs）相结合，制备了红细胞膜仿生光学纳米载体。具有超长近红外持续发光（ZGGO）的三重掺杂锌镓锗酸盐纳米结构用作光学发射中心，包覆在 PLNPs 上的介孔硅（ZGGO@mSiO）用于药物输送，并且引入红细胞膜囊泡进行仿生功能化，以确保开发的纳米载体绕过巨噬细胞摄取和系统清除。由于天然红细胞膜及其膜脂质和相关膜蛋白的涂层，所提出的仿生纳米载体具有细胞模拟特性。保留了 PLNPs 核的适用性，有利于体外激发，所开发的 RBC-ZGGO@mSiO 仿生纳米载体表现出强烈的荧光、超长的持续发光、单分散纳米尺寸、红光可更新性和优异的生物相容性。体内小鼠生物成像和生物分布研究表明，载有阿霉素的红细胞膜仿生纳米探针作为长循环生物成像、原位实时监测和药物输送的理想纳米载体。我们相信，基于 PLNPs 的仿生纳米载体为诊断和治疗应用提供了一个有前途的纳米平台。
