School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, People's Republic of China.
Biomed Mater. 2018 Apr 10;13(4):045001. doi: 10.1088/1748-605X/aab3ea.
Spherical porous hydroxyapatite (SHA)/graphene oxide (GO) composites with different GO (w/w) content of 16%, 40%, and 71% have been fabricated through a facile and controllable ultrasonic-assisted method at room temperature. The products were characterized by x-ray diffraction, field emission scanning electron microscopy, thermogravimetric analysis, mechanical testing and biomimetic mineralization. Results showed SHA were covered by GO, and SHA/GO composites had an irregular surface with different degrees of wrinkles. The elastic modulus and hardness of SHA/GO-3 composites were up to 12.45 ± 0.33 GPa and 686.67 ± 26.95 MPa, which indicated that the contents of GO had an effect on SHA/GO composites. And the mechanical properties of SHA/GO-2 composites were similar to SHA particles. The biomimetic mineralization in SBF solution showed the bone-like apatite layer on composites surface, which demonstrated that the SHA/GO materials had osseointegration property. Moreover, in vitro cytocompatibility of SHA/GO-2 composites and pure GO were evaluated by cell adhesion and proliferation tests using MC3T3-E1 cells, which demonstrated that the SHA/GO composites can act as a good template for the cells growth and adhesion. These results suggested that the SHA/GO composites will be a promising material for biomedical application.
通过简便可控的室温超声辅助法制备了具有不同 GO（w/w）含量（16%、40%和 71%）的球形多孔羟基磷灰石（SHA）/氧化石墨烯（GO）复合材料。采用 X 射线衍射、场发射扫描电子显微镜、热重分析、力学性能测试和仿生矿化对产物进行了表征。结果表明，GO 覆盖在 SHA 上，SHA/GO 复合材料表面具有不同程度褶皱的不规则形貌。SHA/GO-3 复合材料的弹性模量和硬度分别高达 12.45 ± 0.33 GPa 和 686.67 ± 26.95 MPa，表明 GO 的含量对 SHA/GO 复合材料有影响。而 SHA/GO-2 复合材料的力学性能与 SHA 颗粒相似。在 SBF 溶液中的仿生矿化在复合材料表面形成了类骨磷灰石层，表明 SHA/GO 材料具有骨整合性能。此外，通过 MC3T3-E1 细胞黏附和增殖试验评估了 SHA/GO-2 复合材料和纯 GO 的体外细胞相容性，结果表明 SHA/GO 复合材料可以作为细胞生长和黏附的良好模板。这些结果表明，SHA/GO 复合材料将成为一种有前途的生物医学应用材料。
