Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, ‡The Research Center of Biomedical Nanotechnology, and §Key Lab of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University , Lanzhou 730000, P. R. China.
ACS Appl Mater Interfaces. 2014 Mar 12;6(5):3132-40. doi: 10.1021/am4057826. Epub 2014 Feb 24.
In bone tissue engineering, it is imperative to design multifunctional biomaterials that can induce and assemble bonelike apatite that is close to natural bone. In this study, graphene oxide (GO) was functionalized by carrageenan. The resulting GO-carrageenan (GO-Car) composite was further used as a substrate for biomimetic and cell-mediated mineralization of hydroxyapatite (HA). It was confirmed that carrageenan on the GO surface facilitated the nucleation of HA. The observation of the effect of the GO-Car on the adhesion, morphology, and proliferation of MC3T3-E1 cells was investigated. In vitro studies clearly show the effectiveness of GO-Car in promoting HA mineralization and cell differentiation. The results of this study suggested that the GO-Car hybrid will be a promising material for bone regeneration and implantation.
在骨组织工程中，设计能够诱导和组装类似于天然骨的多官能生物材料至关重要。在本研究中，通过卡拉胶对氧化石墨烯（GO）进行了功能化。所得的 GO-卡拉胶（GO-Car）复合物进一步用作仿生和细胞介导的羟基磷灰石（HA）矿化的基底。证实了 GO 表面上的卡拉胶促进了 HA 的成核。研究了 GO-Car 对 MC3T3-E1 细胞黏附、形态和增殖的影响。体外研究清楚地表明了 GO-Car 在促进 HA 矿化和细胞分化方面的有效性。本研究结果表明，GO-Car 杂化材料有望成为骨再生和植入的理想材料。
