Garbacz Katarzyna, Piechowicz Lidia, Podkowik Magdalena, Mroczkowska Aneta, Empel Joanna, Bania Jacek
Department of Oral Microbiology, Medical University of Gdansk, Gdansk, Poland.
Department of Medical Microbiology, Medical University of Gdansk, Gdansk, Poland.
Infect Drug Resist. 2018 Feb 22;11:247-255. doi: 10.2147/IDR.S153427. eCollection 2018.
The aim of this study was to assess the relatedness of molecular types of isolates colonizing cystic fibrosis (CF) patients with their antimicrobial resistance and prevalence of toxin genes.
A total of 215 isolates from the airways of 107 patients with CF were tested for and SCC type, antimicrobial resistance and carriage of toxin genes.
t015, t084, t091, t700 and t002 were the largest group (approximately 25%) among all 69 identified spa types. Five new spa types, t14286, t14287, t14288, t14289 and t14290, were identified and registered. Isolates from CF patients were clustered into 11 multi-locus sequence typing clonal complexes, with CC30, CC22, CC97, CC45, CC15 and CC5 being the most frequent ones. Twelve (5.6%) methicillin-resistant (MRSA) isolates and 102 (47.7%) multidrug-resistant isolates were identified, along with three SCC types (I, III and V). All isolates (both MRSA and methicillin-sensitive ) were Panton-Valentine leucocidin-negative, and 56.7% harbored egc genes. This was the first study documenting the presence of ST398-V-t571 livestock-associated MRSA in a European patient with CF.
These findings imply that individuals with CF can also be colonized with animal-related ST398 MRSA, and justify constant monitoring of staphylococcal colonization and identification of epidemic clones in this group.
本研究旨在评估定植于囊性纤维化（CF）患者的分离株分子类型与其抗菌药物耐药性及毒素基因流行情况之间的相关性。
对107例CF患者气道中的215株分离株进行spa型和SCC型、抗菌药物耐药性及毒素基因携带情况检测。
在所有鉴定出的69种spa型中，t015、t084、t091、t700和t002是最大的一组（约25%）。鉴定并登记了5种新的spa型，即t14286、t14287、t14288、t14289和t14290。CF患者的分离株被聚类为11个多位点序列分型克隆复合体，其中CC30、CC22、CC97、CC45、CC15和CC5最为常见。鉴定出12株（5.6%）耐甲氧西林金黄色葡萄球菌（MRSA）分离株和102株（47.7%）多重耐药分离株，以及3种SCC型（I、III和V）。所有分离株（包括MRSA和甲氧西林敏感金黄色葡萄球菌）均为杀白细胞素阴性型，56.7%携带egc基因。这是首次在欧洲CF患者中记录到ST398-V-t571型与家畜相关的MRSA。
这些发现表明CF患者也可能被与动物相关的ST398 MRSA定植，证明了对该群体中葡萄球菌定植情况进行持续监测以及鉴定流行克隆的合理性。
