Mamelle Elisabeth, El Kechai Naila, Adenis Victor, Nguyen Yann, Sterkers Olivier, Agnely Florence, Bochot Amélie, Edeline Jean Marc, Ferrary Evelyne
a Sorbonne Université, Université Pierre et Marie Curie Paris 6, Inserm, Unité "Réhabilitation Chirurgicale Mini-Invasive et Robotisée de l'Audition" , Paris , France.
b Department of Otolaryngology, Unit of Otology, Auditory Implants and Skull Base Surgery , AP-HP, GH Pitié-Salpêtrière , Paris , France.
Acta Otolaryngol. 2018 Jul;138(7):610-616. doi: 10.1080/00016489.2018.1438659. Epub 2018 Mar 5.
Intratympanic injection of corticosteroids membrane after noise-induced hearing loss is an accepted alternative to general administration. We investigated the effect on hearing of a hyaluronic acid gel with liposomes loaded with dexamethasone (DexP) administered into the middle ear.
An acute acoustic trauma was performed to 13 guinea pigs for a period of 1 h on Day -2. Two 2 days after the noise trauma, the animals were then assigned randomly to four experimental groups: control without gel, gel injection, gel-containing free DexP, gel-containing DexP loaded into liposomes. Auditory thresholds were measured with Auditory Brainstem Response before Day -2 and at Day 0, Day 7 and Day 30 after noise trauma.
Seven days after, a complete hearing recovery was observed in the control group at all frequencies apart from 8 kHz, and no recovery was observed in the three groups receiving a gel injection. Thirty days after trauma, all of the animals had recovered normal hearing, apart from at the 8-kHz frequency, with similar auditory thresholds.
Local DexP administration 48 h after a mild acoustic trauma did not improve hearing recovery, even with a sustained release in a specific gel formulation designed for inner ear therapy.
噪声性听力损失后鼓室内注射皮质类固醇膜是一种被认可的替代全身给药的方法。我们研究了将载有地塞米松的脂质体透明质酸凝胶（DexP）注入中耳对听力的影响。
在第-2天对13只豚鼠进行1小时的急性声创伤。噪声创伤后2天，将动物随机分为四个实验组：无凝胶对照组、凝胶注射组、含游离地塞米松的凝胶组、载有地塞米松的脂质体凝胶组。在第-2天之前以及噪声创伤后的第0天、第7天和第30天，用听觉脑干反应测量听觉阈值。
7天后，除8kHz外，对照组在所有频率上均观察到完全听力恢复，而接受凝胶注射的三组均未观察到恢复。创伤后30天，除8kHz频率外，所有动物均恢复了正常听力，听觉阈值相似。
轻度声创伤48小时后局部给予地塞米松，即使采用专为内耳治疗设计的特定凝胶制剂进行缓释，也不能改善听力恢复。
