Université de Monastir, Faculté des Sciences de Monastir, Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Bd. de l'environnement, 5019 Monastir, Tunisia.
Laboratoire des Plantes Aromatiques et Médicinales (LPAM), Centre de Biotechnologie de Borj- Cédria, BP 901, 2050 Hammam-lif, Tunisia.
Int J Biol Macromol. 2018 Jul 1;113:774-782. doi: 10.1016/j.ijbiomac.2018.03.008. Epub 2018 Mar 2.
Central composite design was performed to optimize uronic acid rate, esterification degree, total antioxidant ability and antiglycation capacity of carbohydrates from Arthrocnemum indicum leaves. Three independent variables were opted: extraction temperature, time and ratio (solvent/material). The optimal settings were: extraction temperature of 80°C, time of 288min and (solvent/solid) ratio of 40mL/g. Under these settings, uronic acid rate and esterification degree were 49.29%, 30.24%, respectively, whereas total antioxidant activity and antiglycation capacity was 35.81mg ascorbic acid equivalents/g matter and 69.81%, respectively. Colorimetric assays showed that total sugar and uronic acid contents for polysaccharide were 71.78% and 49.24%, respectively. Furthermore, Preliminary structure study was performed via various methods including FT-IR, NMR and UV-vis analysis. SEC analyzes revealed that polysaccharide had an average molecular weight of 2179kDa. Moreover, GC-MS analyzes showed that extracted polysaccharide was a pectic polysaccharide which formed of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 66.68%, 3.93%, 12.71%, 6.31%, 6.08% and 4.29%, respectively. This results revealed that extracted polysaccharide can be employed as source of natural antioxidants and as possible antiglycated agents.
采用中心组合设计优化了节旋木层孔菌叶中碳水化合物的糖醛酸含量、酯化度、总抗氧化能力和糖化抑制能力。选择了三个独立变量：提取温度、时间和比例（溶剂/材料）。最佳设置为：提取温度 80°C，时间 288min，（溶剂/固体）比为 40mL/g。在此设定下，糖醛酸含量和酯化度分别为 49.29%和 30.24%，而总抗氧化活性和糖化抑制能力分别为 35.81mg 抗坏血酸当量/g 物质和 69.81%。比色法测定表明，多糖的总糖和糖醛酸含量分别为 71.78%和 49.24%。此外，通过各种方法（包括 FT-IR、NMR 和 UV-vis 分析）进行了初步的结构研究。SEC 分析表明，多糖的平均分子量为 2179kDa。此外，GC-MS 分析表明，提取的多糖是一种果胶多糖，由阿拉伯糖、甘露糖、半乳糖、鼠李糖、葡萄糖和木糖组成，摩尔百分比分别为 66.68%、3.93%、12.71%、6.31%、6.08%和 4.29%。这些结果表明，提取的多糖可用作天然抗氧化剂和可能的糖化抑制剂的来源。
