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从天然抗氧化剂和抗炎剂中提取多糖的酶法提取、纯化和特性研究。

Enzymatic Extraction, Purification, and Characterization of Polysaccharides from : Natural Antioxidant and Anti-Inflammatory.

机构信息

College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.

Collaborative Innovation Center for the Protection and Utilization of Chinese Herbal Medicine Resources in Hunan Province, Hunan University of Chinese Medicine, Changsha 410208, China.

出版信息

Biomed Res Int. 2018 Nov 27;2018:3486864. doi: 10.1155/2018/3486864. eCollection 2018.

DOI:10.1155/2018/3486864
PMID:30598992
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6288581/
Abstract

(PCP) is a kind of functional food or medicine for liver protection. In the present work, Plackett-Burman design, steepest ascent method, and response surface methodology (RSM) were employed to obtain maximum total sugar yield. The experimental yield of 6.91% indicated a close agreement with the predicted yield of 7.00% of the model under optimized conditions. The major polysaccharide fraction (PCPP-1a) from PCPP was purified and identified as acidic polysaccharides with a high content of uronic acid (FT-IR, UV, HPGPC). PCPP had similar monosaccharide profile with PCPP-1a but was rich in galacturonic acid (HPLC). Both of PCPP and PCPP-1a possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe chelating activities. Moreover, they were revealed to show strong anti-inflammatory activities by inhibiting NO, TNF-, and IL-1 release compared to LPS treatment in RAW264.7 cells. These data suggest that the polysaccharides from PCP could be potential natural products for treating ROS and inflammatory-related diseases.

摘要

（PCP）是一种具有保肝作用的功能性食品或药物。本研究采用 Plackett-Burman 设计、最陡爬坡法和响应面法（RSM）来获得最大的总糖得率。实验得出的 6.91%的产率与模型优化条件下预测的 7.00%的产率非常吻合。PCPP 的主要多糖组分（PCPP-1a）经过纯化和鉴定，为酸性多糖，含有较高的糖醛酸（FT-IR、UV、HPGPC）。PCPP 的单糖组成与 PCPP-1a 相似，但富含半乳糖醛酸（HPLC）。PCPP 和 PCPP-1a 均具有较强的羟基自由基清除、DPPH 自由基清除和 Fe 螯合活性。此外，与 LPS 处理 RAW264.7 细胞相比，它们通过抑制 NO、TNF-α 和 IL-1 的释放，表现出较强的抗炎活性。这些数据表明，PCP 中的多糖可能是治疗 ROS 和炎症相关疾病的潜在天然产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/f82563c2709f/BMRI2018-3486864.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/9e8d346981f6/BMRI2018-3486864.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/60009b6daa0d/BMRI2018-3486864.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/15cf14f793bf/BMRI2018-3486864.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/7ca2409a7a2b/BMRI2018-3486864.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/8d4761ee7ebc/BMRI2018-3486864.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/f82563c2709f/BMRI2018-3486864.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/9e8d346981f6/BMRI2018-3486864.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/60009b6daa0d/BMRI2018-3486864.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/15cf14f793bf/BMRI2018-3486864.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/7ca2409a7a2b/BMRI2018-3486864.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/8d4761ee7ebc/BMRI2018-3486864.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b0e0/6288581/f82563c2709f/BMRI2018-3486864.006.jpg

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