Chinese PLA Institute for Disease Control and Prevention, Beijing, China.
Dermatology Department, Tongji Hospital of Tongji University, Shanghai, China.
Antimicrob Agents Chemother. 2018 Apr 26;62(5). doi: 10.1128/AAC.01549-17. Print 2018 May.
The use of azole fungicides in agriculture is believed to be one of the main reasons for the emergence of azole resistance in Though widely used in agriculture, imidazole fungicides have not been linked to resistance in This study showed that elevated MIC values of imidazole drugs were observed against isolates with TR/L98H/S297T/F495I mutation, but not among isolates with TR/L98H mutation. Short-tandem-repeat (STR) typing analysis of 580 isolates from 20 countries suggested that the majority of TR/L98H/S297T/F495I strains from China were genetically different from the predominant major clade comprising most of the azole-resistant strains and the strains with the same mutation from the Netherlands and Denmark. Alignments of sterol 14α-demethylase sequences suggested that F495I in was orthologous to F506I in and F489L in , which have been reported to be associated with imidazole resistance. antifungal susceptibility testing of different recombinants with mutations further confirmed the association of the F495I mutation with imidazole resistance. In conclusion, this study suggested that environmental use of imidazole fungicides might confer selection pressure for the emergence of azole resistance in .
农业中唑类杀真菌剂的使用被认为是棘白菌素类耐药性出现的主要原因之一。尽管咪唑类杀真菌剂在农业中广泛使用，但它们与耐药性无关。本研究表明，具有 TR/L98H/S297T/F495I 突变的 分离株对咪唑类药物的 MIC 值升高，但具有 TR/L98H 突变的分离株则没有。来自 20 个国家的 580 株 的短串联重复（STR）分型分析表明，来自中国的大多数 TR/L98H/S297T/F495I 菌株在遗传上与主要的优势分支不同，该分支包含了大多数棘白菌素类耐药菌株以及来自荷兰和丹麦的具有相同突变的菌株。甾醇 14α-脱甲基酶序列的比对表明， 中的 F495I 与 中的 F506I 和 中的 F489L 是同源的，这两者已被报道与咪唑类耐药性有关。不同具有 突变的重组体的抗真菌药敏试验进一步证实了 F495I 突变与咪唑类耐药性的相关性。总之，本研究表明，咪唑类杀真菌剂在环境中的使用可能会对 的棘白菌素类耐药性的出现产生选择压力。
