A Whole Genome Sequencing-Based Approach to Track down Genomic Variants in Itraconazole-Resistant Species of from Iran.

The antifungal resistance in non-fumigatus spp., as well as , poses a major therapeutic challenge which affects the entire healthcare community. Mutation occurrence of 51 gene paralogs is the major cause of azole resistance in spp. To obtain a full map of genomic changes, an accurate scan of the entire length of the genome is necessary. In this study, using whole genome sequencing (WGS) technique, we evaluated the mutation in , , and genes in different clinical isolates of , , , and which responded to minimum inhibitory concentrations of itraconazole above 16 µg mL. We found different nonsynonymous mutations in the , , and gene loci. According to our findings, species isolated from different parts of the world may represent different pattern of resistance mechanisms which may be revealed by WGS.