Escobedo-Hinojosa Wendy Itzel, Gomez-Chang Erika, García-Martínez Karina, Guerrero Alquicira Raquel, Cardoso-Taketa Alexandre, Romero Irma
Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 Ciudad de México, Mexico.
Departamento de Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510 Ciudad de México, Mexico.
Evid Based Complement Alternat Med. 2018 Jan 9;2018:2862706. doi: 10.1155/2018/2862706. eCollection 2018.
Gastric ulcers are a worldwide health problem and their poor healing is one of the most important causes for their recurrence. We have previously reported the remarkable gastroprotective and anti- activities of the methanolic extract (CpMet) of bark. This work investigates, in a murine model, the CpMet gastroprotective mechanism and establishes its preclinical efficacy in the resolution of ethanol-induced gastric ulcers. The results showed that the gastroprotective activity of CpMet is mainly associated with endogenous NO and prostaglandins, followed by sulfhydryl groups and K channels. Furthermore, CpMet (300 mg/kg, twice a day) orally administered during 20 consecutive days promoted an ulcer area reduction of 62.65% at the 20th day of the treatment. The effect was confirmed macroscopically by the alleviation of gastric mucosal erosions and microscopically by an increase in mucin content and a reduction in the inflammatory infiltration at the site of the ulcer. No clinical symptoms or signs of toxicity were observed in the treated animals. The results indicate the safety and efficacy of CpMet in promoting high quality of ulcer healing by different mechanisms, but mostly through cytoprotective and anti-inflammatory effects, making it a promising phytodrug for ulcer treatment.
胃溃疡是一个全球性的健康问题，其愈合不良是复发的最重要原因之一。我们之前报道过[植物名称]树皮甲醇提取物（CpMet）具有显著的胃保护和抗炎活性。这项研究在小鼠模型中探究了CpMet的胃保护机制，并确定了其在治疗乙醇诱导的胃溃疡方面的临床前疗效。结果表明，CpMet的胃保护活性主要与内源性一氧化氮和前列腺素有关，其次是巯基和钾通道。此外，连续20天每天两次口服给予CpMet（300mg/kg），在治疗第20天时溃疡面积减少了62.65%。从宏观上看，胃黏膜糜烂减轻证实了这一效果；从微观上看，溃疡部位的粘蛋白含量增加和炎症浸润减少也证实了这一效果。在接受治疗的动物中未观察到任何临床症状或毒性迹象。结果表明，CpMet通过不同机制促进高质量溃疡愈合具有安全性和有效性，但其主要作用机制是细胞保护和抗炎作用，这使其成为一种有前景的用于溃疡治疗的植物药。
