Centre for Soybean Research, Partner State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong Special Administrative Region, China.
Plant Cell Environ. 2019 Jan;42(1):98-114. doi: 10.1111/pce.13186. Epub 2018 Jun 1.
To obtain a comprehensive understanding of transcriptomic reprogramming under salt stress, we performed whole-transcriptome sequencing on the leaf and root of soybean seedlings subjected to salt treatment in a time-course experiment (0, 1, 2, 4, 24, and 48 hr). This time series dataset enabled us to identify important hubs and connections of gene expressions. We highlighted the analysis on phytohormone signaling pathways and their possible crosstalks. Differential expressions were also found among those genes involved in carbon and nitrogen metabolism. In general, the salt-treated seedlings slowed down their photosynthetic functions and ramped up sugar catabolism to provide extra energy for survival. Primary nitrogen assimilation was shut down whereas nitrogen resources were redistributed. Overall, the results from the transcriptomic analyses indicate that the plant uses a multipronged approach to overcome salt stress, with both fast-acting, immediate physiological responses, and longer term reactions that may involve metabolic adjustment.
为了全面了解盐胁迫下的转录组重编程，我们对经过盐处理的大豆幼苗的叶片和根部进行了时间序列实验（0、1、2、4、24 和 48 小时）的全转录组测序。这个时间序列数据集使我们能够识别基因表达的重要枢纽和连接。我们重点分析了植物激素信号通路及其可能的串扰。我们还发现了参与碳氮代谢的基因之间的差异表达。总的来说，盐处理的幼苗减缓了它们的光合作用功能，并加速了糖的分解代谢，为生存提供额外的能量。初级氮同化被关闭，而氮资源被重新分配。总的来说，转录组分析的结果表明，植物采用了多管齐下的方法来克服盐胁迫，既有快速、即时的生理反应，也有涉及代谢调整的长期反应。
