MOE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering , Zhejiang University , Hangzhou 310027 , China.
ACS Appl Mater Interfaces. 2018 Mar 28;10(12):10428-10436. doi: 10.1021/acsami.7b18889. Epub 2018 Mar 15.
Cationic antibacterial coating based on quaternary ammonium compounds, with an efficient and broad spectrum bactericidal property, has been widely used in various fields. However, the high density of positive charges tends to induce weak hemocompatibility, which hinders the application of the cationic antibacterial coating in blood-contacting devices and implants. It has been reported that a negatively charged surface can reduce blood coagulation, showing improved hemocompatibility. Here, we describe a strategy to combine the cationic and anionic groups by using mixed-charged copolymers. The copolymers of poly (quaternized vinyl pyridine- co- n-butyl methacrylate- co-methacrylate acid) [P(QVP- co- nBMA- co-MAA)] were synthesized through free radical copolymerization. The cationic group of QVP, the anionic group of MAA, and the hydrophobic group of nBMA were designed to provide bactericidal capability, hemocompatibility, and coating stability, respectively. Our findings show that the hydrophilicity of the copolymer coating increased, and its zeta potential decreased from positive charge to negative charge with the increase of the anionic/cationic ratio. Meanwhile, the bactericidal property of the copolymer coating was kept around a similar level compared with the pure quaternary ammonium copolymer coating. Furthermore, the coagulation time, platelet adhesion, and hemolysis tests revealed that the hemocompatibility of the copolymer coating improved with the addition of the anionic group. The mixed-charged copolymer combined both bactericidal property and hemocompatibility and has a promising potential in blood-contacting antibacterial devices and implants.
基于季铵盐的阳离子抗菌涂层具有高效广谱的杀菌性能，已广泛应用于各个领域。然而，高浓度的正电荷往往会导致弱的血液相容性，这阻碍了阳离子抗菌涂层在与血液接触的设备和植入物中的应用。据报道，带负电荷的表面可以减少血液凝固，表现出更好的血液相容性。在这里，我们描述了一种通过使用混合带电共聚物来结合阳离子和阴离子基团的策略。通过自由基共聚合成了聚（季铵化乙烯基吡啶-共-正丁基甲基丙烯酸酯-共-甲基丙烯酸）[P（QVP-co-nBMA-co-MAA）]共聚物。QVP 的阳离子基团、MAA 的阴离子基团和 nBMA 的疏水性基团分别设计用于提供杀菌能力、血液相容性和涂层稳定性。我们的研究结果表明，随着阴离子/阳离子比例的增加，共聚物涂层的亲水性增加，其 zeta 电位从正电荷变为负电荷。同时，与纯季铵盐共聚物涂层相比，共聚物涂层的杀菌性能保持在相似水平。此外，凝血时间、血小板黏附及溶血试验表明，随着阴离子基团的加入，共聚物涂层的血液相容性得到改善。这种混合带电共聚物结合了杀菌性能和血液相容性，在与血液接触的抗菌设备和植入物中有很好的应用前景。
