Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Bio-functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, P. R. China.
J Mater Chem B. 2021 Oct 13;9(39):8321-8329. doi: 10.1039/d1tb01676h.
Designing a coating material with efficient bactericidal property to cope with bacterial associated infections is highly desirable for metallic implants and devices. Here, we report phosphonate/quaternary ammonium copolymers, (DEMMP--TMAEMA), as the new type of metal anchorable high-efficiency antibacterial coating. Seven (DEMMP--TMAEMA) polymers with varied cationic components were precisely prepared random radical polymerization. Copolymers were constructed on titanium alloy (TC4) substrates based on strong covalent bonding between the phosphonate group and metallic substrates through a one-step process as evidenced by XPS and water contact angle tests. A robust relationship between the composition of the copolymers and the bactericidal ability endowed to TC4 substrates was established. Results showed that the copolymer, with the DEMMP content even as low as 6.3%, was able to anchor onto TC4 substrates. With the increase of cationic TMAEMA content from 4.0 to 93.7% in the coating copolymer, the bactericidal ability endowed to the TC4 substrates was steadily increased from 39.4 to 98.8% for and from 70.0 to 99.4% for after 8 h's of contacting. All (DEMMP--TMAEMA) coating on TC4 substrates showed limited cytotoxicity to C2C12 cells. Notably, the phosphonate/quaternary amine copolymers can be easily constructed on diverse biomedical metals such as titanium (Ti), stainless steel (SS), and Ni/Cr alloys with significantly increased antibacterial performance, demonstrating the potency of the copolymer as the general high-efficiency antibacterial coating for diverse bio-metals.
设计具有高效杀菌性能的涂层材料来应对与细菌相关的感染，对于金属植入物和设备来说是非常理想的。在这里，我们报告了膦酸酯/季铵盐共聚物（DEMMP--TMAEMA），作为新型金属锚定高效抗菌涂层。通过自由基聚合精确制备了 7 种带有不同阳离子组分的（DEMMP--TMAEMA）共聚物。共聚物通过一步法构建在钛合金（TC4）基底上，这是通过 XPS 和水接触角测试证明的，其中膦酸酯基团与金属基底之间存在强共价键。共聚物的组成与赋予 TC4 基底的杀菌能力之间建立了牢固的关系。结果表明，即使共聚物中的 DEMMP 含量低至 6.3%，也能够锚定在 TC4 基底上。随着涂层共聚物中阳离子 TMAEMA 含量从 4.0%增加到 93.7%，赋予 TC4 基底的杀菌能力从 39.4%稳步增加到 98.8%（对于 ）和从 70.0%增加到 99.4%（对于 ），接触 8 小时后。所有（DEMMP--TMAEMA）涂层在 TC4 基底上对 C2C12 细胞的细胞毒性有限。值得注意的是，膦酸酯/季铵盐共聚物可以很容易地构建在多种生物医学金属上，如钛（Ti）、不锈钢（SS）和 Ni/Cr 合金，具有显著提高的抗菌性能，证明了共聚物作为通用高效抗菌涂层在多种生物金属上的潜力。
