Yániz J L, Silvestre M A, Santolaria P, Soler C
TECNOGAM Research Group, Environmental Sciences Institute (IUCA), Department of Animal Production and Food Sciences, University of Zaragoza, 22071, Huesca, Spain.
Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia, 46100, Burjassot, Valencia, Spain.
Reprod Fertil Dev. 2018 Jun;30(6):799-809. doi: 10.1071/RD17432.
Sperm motility is one of the most widely used parameters of sperm quality. Computer-aided sperm motility analysis (CASA-Mot) systems were developed to reduce the subjectivity of sperm motility assessment, and have had broad scientific and practical acceptance. In this review, the sources of variation and current applications of this technology and its relationships with other sperm quality tests are described in detail. Despite remarkable advances in the technique, there is still great need for standardisation in many species, and the numerous factors that affect the results make it difficult to provide universally accepted criteria for classifying semen samples based on sperm motility characteristics. The main fields for CASA-Mot include the study of male fertility and pathologies, evaluation of the effects of physical and chemical agents, improvement of epidemiological survey studies, more precise calculation of seminal doses for farm animals, realisation of basic studies about sperm function, improvement of sperm technologies such as cryopreservation and quality control analysis. Numerous relationships have been established between CASA-Mot and other sperm quality tests, although most of these parameters are complementary. Future CASA-Mot systems will probably be able to integrate several sperm quality parameters with motility.
精子活力是精子质量最广泛使用的参数之一。计算机辅助精子活力分析（CASA-Mot）系统的开发旨在减少精子活力评估的主观性，并已获得广泛的科学和实际认可。在本综述中，详细描述了该技术的变异来源、当前应用及其与其他精子质量检测的关系。尽管该技术取得了显著进展，但许多物种仍非常需要标准化，而且影响结果的众多因素使得难以提供基于精子活力特征对精液样本进行分类的普遍接受的标准。CASA-Mot的主要应用领域包括男性生育力和病理学研究、物理和化学因素影响的评估、流行病学调查研究的改进、农场动物精液剂量的更精确计算、精子功能基础研究的开展、冷冻保存等精子技术的改进以及质量控制分析。CASA-Mot与其他精子质量检测之间已经建立了许多联系，尽管这些参数大多是互补的。未来的CASA-Mot系统可能能够将多个精子质量参数与活力整合在一起。
