Bompart Daznia, García-Molina Almudena, Valverde Anthony, Caldeira Carina, Yániz Jesús, Núñez de Murga Manuel, Soler Carles
Proiser R+D, Avenuenida Catedrático Agustín Escardino, 9, Building 3 (CUE), Floor 1, 46980, Paterna, Spain.
University of Valencia, Department of Cellular Biology, Functional Biology and Physical Anthropology, Campus Burjassot, C/ Dr Moliner, 50, 46100, Burjassot, Spain.
Reprod Fertil Dev. 2018 Jun;30(6):810-819. doi: 10.1071/RD17551.
For over 30 years, CASA-Mot technology has been used for kinematic analysis of sperm motility in different mammalian species, but insufficient attention has been paid to the technical limitations of commercial computer-aided sperm analysis (CASA) systems. Counting chamber type and frame rate are two of the most important aspects to be taken into account. Counting chambers can be disposable or reusable, with different depths. In human semen analysis, reusable chambers with a depth of 10µm are the most frequently used, whereas for most farm animal species it is more common to use disposable chambers with a depth of 20µm . The frame rate was previously limited by the hardware, although changes in the number of images collected could lead to significant variations in some kinematic parameters, mainly in curvilinear velocity (VCL). A frame rate of 60 frames s-1 is widely considered to be the minimum necessary for satisfactory results. However, the frame rate is species specific and must be defined in each experimental condition. In conclusion, we show that the optimal combination of frame rate and counting chamber type and depth should be defined for each species and experimental condition in order to obtain reliable results.
30多年来，CASA-Mot技术一直用于不同哺乳动物物种精子活力的运动学分析，但商业计算机辅助精子分析（CASA）系统的技术局限性却未得到足够重视。计数室类型和帧率是需要考虑的两个最重要方面。计数室可以是一次性的或可重复使用的，深度也各不相同。在人类精液分析中，最常用的是深度为10μm的可重复使用计数室，而对于大多数家畜物种，更常用的是深度为20μm的一次性计数室。帧率以前受硬件限制，尽管采集图像数量的变化可能导致一些运动学参数出现显著变化，主要是曲线速度（VCL）。普遍认为60帧·秒-1的帧率是获得满意结果所需的最低帧率。然而，帧率因物种而异，必须在每种实验条件下加以确定。总之，我们表明，为了获得可靠结果，应针对每个物种和实验条件确定帧率、计数室类型和深度的最佳组合。
