Zheng Jian, Ma Xiaohua, Zhang Xule, Hu Qingdi, Qian Renjuan
Institute of Subtropical Crops of Zhejiang Province, 334 Xueshan Road, Wenzhou, 325005 Zhejiang China.
Physiol Mol Biol Plants. 2018 Mar;24(2):231-238. doi: 10.1007/s12298-017-0496-x. Epub 2018 Jan 17.
Salt stress is a critical factor that affects the growth and development of plants. Salicylic acid (SA) is an important signal molecule that mitigates the negative effects of salt stress on plants. To elucidate salt tolerance in large pink L. (Caryophyllaceae) and the regulatory mechanism of exogenous SA on under different salt stresses, we conducted a pot experiment to evaluate leaf biomass, leaf anatomy, soluble protein and sugar content, and the relative expression of salt-induced genes in under 0.3, 0.6, and 0.9% NaCl conditions with and without 0.5 mM SA. The result showed that exposure of to salt stress lead to a decrease in leaf growth, soluble protein and sugar content, and mesophyll thickness, together with an increase in the expression of and genes. Foliar application of SA effectively increased leaf biomass, soluble protein and sugar content, and upregulated the expression of and in the , which facilitated in the acclimation of to moderate salt stress. However, when the plants were grown under severe salt stress (0.9% NaCl), no significant difference in plant physiological responses and relevant gene expression between plants with and without SA was observed. The findings of this study suggest that exogenous SA can effectively counteract the adverse effects of moderate salt stress on growth and development.
盐胁迫是影响植物生长发育的关键因素。水杨酸（SA）是一种重要的信号分子，可减轻盐胁迫对植物的负面影响。为了阐明大花剪秋罗（石竹科）的耐盐性以及外源SA在不同盐胁迫下对其的调控机制，我们进行了盆栽试验，以评估在0.3%、0.6%和0.9% NaCl条件下，添加和不添加0.5 mM SA时大花剪秋罗的叶片生物量、叶片解剖结构、可溶性蛋白和糖含量，以及盐诱导基因的相对表达。结果表明，大花剪秋罗受到盐胁迫会导致叶片生长、可溶性蛋白和糖含量以及叶肉厚度降低，同时和基因的表达增加。叶面喷施SA有效增加了叶片生物量、可溶性蛋白和糖含量，并上调了大花剪秋罗中和的表达，这有助于大花剪秋罗适应中度盐胁迫。然而，当植物在重度盐胁迫（0.9% NaCl）下生长时，喷施SA和未喷施SA的植物在生理反应和相关基因表达上没有显著差异。本研究结果表明，外源SA可以有效对抗中度盐胁迫对大花剪秋罗生长发育的不利影响。
