Zhao Jieru, Han Qibiao, Cui Bingjian, Wang Juan, Hu Chao, Li Rui, Lin Yanyu, Xu Ying, Liu Chuncheng
College of Hydraulic Science and Engineering, Yangzhou University, Yangzhou 225009, China.
Institute of Farmland Irrigation, Chinese Academy of Agricultural Sciences, Xinxiang 453002, China.
Plants (Basel). 2025 Jul 6;14(13):2065. doi: 10.3390/plants14132065.
Salinization severely impairs crop growth by inducing oxidative stress and disrupting cellular homeostasis. This study systematically investigates the synergistic effects of salt-tolerant plant-growth-promoting rhizobacteria (ST-PGPR) and foliar silicon fertilizer spraying (FSFS) on antioxidant responses in Pak choi under salt stress. Two-season pot experiments were carried out to evaluate key indicators, including antioxidant enzyme activities (superoxide dismutase: SOD; peroxidase: POD; catalase: CAT), oxidative stress (malondialdehyde: MDA), osmolyte accumulation (proline, soluble protein), and hormones (Jasmonic Acid: JA; Salicylic Acid: SA; Abscisic acid: ABA). The results demonstrate that combining ST-PGPR with FSFS significantly enhances SOD (6.18-2353.85%), POD (3.44-153.29%), and CAT (25.71-319.29%) activities while reducing MDA content (8.12-35.87%). Proline and soluble protein levels increased by 1.56-15.71% and 5.03-188.87%, respectively. Hormonal regulation increased JA, SA, and ABA levels by 1.05-31.81%, 2.09-34.29%, and 3.18-30.09%, respectively. Notably, ST-PGPR treatments at 10 and 10 cfu·mL, combined with foliar silicon application, consistently ranked highest in overall antioxidant performance across both seasons based on a principal component analysis. These findings provide novel insights into microbial-mineral interactions for sustainable saline agriculture.
盐渍化通过诱导氧化应激和破坏细胞内稳态严重损害作物生长。本研究系统地调查了耐盐促植物生长根际细菌（ST-PGPR）和叶面喷施硅肥（FSFS）对盐胁迫下小白菜抗氧化反应的协同作用。进行了两季盆栽试验，以评估关键指标，包括抗氧化酶活性（超氧化物歧化酶：SOD；过氧化物酶：POD；过氧化氢酶：CAT）、氧化应激（丙二醛：MDA）、渗透调节物质积累（脯氨酸、可溶性蛋白）和激素（茉莉酸：JA；水杨酸：SA；脱落酸：ABA）。结果表明，将ST-PGPR与FSFS结合使用可显著提高SOD（6.18-2353.85%）、POD（3.44-153.29%）和CAT（25.71-319.29%）的活性，同时降低MDA含量（8.12-35.87%）。脯氨酸和可溶性蛋白水平分别提高了1.56-15.71%和5.03-188.87%。激素调节使JA、SA和ABA水平分别提高了1.05-31.81%、2.09-34.29%和3.18-30.09%。值得注意的是，根据主成分分析，10和10 cfu·mL的ST-PGPR处理与叶面施用硅肥相结合，在两个季节的整体抗氧化性能中始终排名最高。这些发现为可持续盐碱农业中的微生物-矿物质相互作用提供了新的见解。
