Cai Jun, Yang Hongxia, Shi Song, Zhong Guohua, Yi Xin
Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China.
Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, China.
Front Physiol. 2018 Feb 20;9:118. doi: 10.3389/fphys.2018.00118. eCollection 2018.
The sterile insect technique (SIT) may reduce pest populations by allowing sufficient amount of irradiation-induced sterile males to mate with wild females whilst maintaining mating ability comparable to wild males. Although the SIT methods are well understood, the optimal sterilizing dose and processing development stage for application vary among species. To ensure effective pest control programs, effects of irradiation on physiology, behavior, and gene function in the target species should be defined, however, little is known about irradiation effects in . Here, the effects of irradiation on rates of fecundity, egg hatch, eclosion, mating competitiveness, flight capability, morphology of reproductive organs, and yolk protein (YP) gene expression were studied. The results showed that rates of female fecundity and egg hatch decreased significantly (51 ± 19 to 0.06 ± 0.06 and 98.90 ± 1.01 to 0, respectively) when pupae were treated with >150 Gy irradiation. Flight capability and mating competitiveness were not significantly influenced at doses <250 Gy. Ovaries and fallopian tubes became smaller after irradiation, but there was no change in testes size. Finally, we found that expression of the gene was up-regulated by irradiation at 30 and 45 days post-emergence, but the mechanisms were unclear. Our study provides information on the determination of the optimal irradiation sterilizing dose in , and the effects of irradiation on physiology, morphology and gene expression that will facilitate an understanding of sub-lethal impacts of the SIT and expand its use to the control of other species.
不育昆虫技术（SIT）可通过使足够数量的经辐射诱导的不育雄虫与野生雌虫交配，同时保持与野生雄虫相当的交配能力，来减少害虫种群数量。尽管SIT方法已广为人知，但应用的最佳绝育剂量和处理发育阶段因物种而异。为确保有效的害虫防治计划，应明确辐射对目标物种生理、行为和基因功能的影响，然而，关于辐射在[具体物种未提及]中的影响却知之甚少。在此，研究了辐射对繁殖力、卵孵化率、羽化率、交配竞争力、飞行能力、生殖器官形态以及卵黄蛋白（YP）基因表达的影响。结果表明，当蛹接受>150 Gy辐射处理时，雌虫繁殖力和卵孵化率显著下降（分别从51±19降至0.06±0.06以及从98.90±1.01降至0）。在剂量<250 Gy时，飞行能力和交配竞争力未受到显著影响。辐射后卵巢和输卵管变小，但睾丸大小没有变化。最后，我们发现[具体基因未提及]基因的表达在羽化后30天和45天时因辐射而上调，但其机制尚不清楚。我们的研究提供了关于确定[具体物种未提及]最佳辐射绝育剂量的信息，以及辐射对生理、形态和基因表达的影响，这将有助于理解SIT的亚致死影响，并将其应用扩展到控制其他物种。
